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DOI | 10.1126/science.aaa3761 |
Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler | |
Sinha, Kalyan K.1; Gross, John D.2; Narlikar, Geeta J.1 | |
2017-01-20 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6322 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Adenosine 5'-triphosphate (ATP)-dependent chromatin remodeling enzymes play essential biological roles by mobilizing nucleosomal DNA. Yet, how DNA is mobilized despite the steric constraints placed by the histone octamer remains unknown. Using methyl transverse relaxation-optimized nuclear magnetic resonance spectroscopy on a 450-kilodalton complex, we show that the chromatin remodeler, SNF2h, distorts the histone octamer. Binding of SNF2h in an activated ATP state changes the dynamics of buried histone residues. Preventing octamer distortion by site-specific disulfide linkages inhibits nucleosome sliding by SNF2h while promoting octamer eviction by the SWI-SNF complex, RSC. Our findings indicate that the histone core of a nucleosome is more plastic than previously imagined and that octamer deformation plays different roles based on the type of chromatin remodeler. Octamer plasticity may contribute to chromatin regulation beyond ATP-dependent remodeling. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000392204800030 |
WOS关键词 | MOLECULAR-WEIGHT PROTEINS ; DNA TRANSLOCATION ; NMR-SPECTROSCOPY ; SWI/SNF COMPLEX ; ISW2-NUCLEOSOME COMPLEX ; ANGSTROM RESOLUTION ; (H3-H4)(2) TETRAMER ; H4 TAIL ; ATP ; ISWI |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195271 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA; 2.Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA |
推荐引用方式 GB/T 7714 | Sinha, Kalyan K.,Gross, John D.,Narlikar, Geeta J.. Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler[J]. SCIENCE,2017,355(6322). |
APA | Sinha, Kalyan K.,Gross, John D.,&Narlikar, Geeta J..(2017).Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.SCIENCE,355(6322). |
MLA | Sinha, Kalyan K.,et al."Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler".SCIENCE 355.6322(2017). |
条目包含的文件 | 条目无相关文件。 |
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