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DOI | 10.1111/gcb.13397 |
Single-cell spatial reconstruction reveals global division of labour in the mammalian liver | |
Halpern, Keren Bahar1; Shenhav, Rom1; Matcovitch-Natan, Orit2; Toth, Beata1; Lemze, Doron1; Golan, Matan1; Massasa, Efi E.1; Baydatch, Shaked1; Landen, Shanie1; Moor, Andreas E.1; Brandis, Alexander3; Giladi, Amir2; Stokar-Avihail, Avigail1; David, Eyal2; Amit, Ido2; Itzkovitz, Shalev1 | |
2017-02-16 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 542期号:7641页码:352-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Israel |
英文摘要 | The mammalian liver consists of hexagon-shaped lobules that are radially polarized by blood flow and morphogens(1-4). Key liver genes have been shown to be differentially expressed along the lobule axis, a phenomenon termed zonation(5,6), but a detailed genome-wide reconstruction of this spatial division of labour has not been achieved. Here we measure the entire transcriptome of thousands of mouse liver cells and infer their lobule coordinates on the basis of a panel of zonated landmark genes, characterized with single-molecule fluorescence in situ hybridization(7). Using this approach, we obtain the zonation profiles of all liver genes with high spatial resolution. We find that around 50% of liver genes are significantly zonated and uncover abundant non-monotonic profiles that peak at the mid-lobule layers. These include a spatial order of bile acid biosynthesis enzymes that matches their position in the enzymatic cascade. Our approach can facilitate the reconstruction of similar spatial genomic blueprints for other mammalian organs. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000394451600038 |
WOS关键词 | ZONAL GENE-EXPRESSION ; MESSENGER-RNA ; ZONATION ; TISSUE ; SEQ ; METABOLISM ; ENERGY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/17396 |
专题 | 气候变化 资源环境科学 |
作者单位 | 1.Weizmann Inst Sci, Dept Mol Cell Biol, Rehovot, Israel; 2.Weizmann Inst Sci, Dept Immunol, Rehovot, Israel; 3.Weizmann Inst Sci, Biol Serv, Rehovot, Israel |
推荐引用方式 GB/T 7714 | Halpern, Keren Bahar,Shenhav, Rom,Matcovitch-Natan, Orit,et al. Single-cell spatial reconstruction reveals global division of labour in the mammalian liver[J]. NATURE,2017,542(7641):352-+. |
APA | Halpern, Keren Bahar.,Shenhav, Rom.,Matcovitch-Natan, Orit.,Toth, Beata.,Lemze, Doron.,...&Itzkovitz, Shalev.(2017).Single-cell spatial reconstruction reveals global division of labour in the mammalian liver.NATURE,542(7641),352-+. |
MLA | Halpern, Keren Bahar,et al."Single-cell spatial reconstruction reveals global division of labour in the mammalian liver".NATURE 542.7641(2017):352-+. |
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