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Plant 22-nt siRNAs mediate translational repression and stress adaptation 期刊论文
NATURE, 2020, 581 (7806) : 89-+
作者:  Roulis, Manolis;  Kaklamanos, Aimilios;  Schernthanner, Marina;  Bielecki, Piotr;  Zhao, Jun;  Kaffe, Eleanna;  Frommelt, Laura-Sophie;  Qu, Rihao;  Knapp, Marlene S.;  Henriques, Ana;  Chalkidi, Niki;  Koliaraki, Vasiliki;  Jiao, Jing;  Brewer, J. Richard;  Bacher, Maren;  Blackburn, Holly N.;  Zhao, Xiaoyun;  Breyer, Richard M.;  Aidinis, Vassilis;  Jain, Dhanpat;  Su, Bing;  Herschman, Harvey R.;  Kluger, Yuval;  Kollias, George;  Flavell, Richard A.
收藏  |  浏览/下载:32/0  |  提交时间:2020/07/03

Characterization of 22-nucleotide short interfering RNAs in plants finds that they accumulate in response to environmental stress, causing translational repression, inhibition of plant growth and enhanced stress responses.


Small interfering RNAs (siRNAs) are essential for proper development and immunity in eukaryotes(1). Plants produce siRNAs with lengths of 21, 22 or 24 nucleotides. The 21- and 24-nucleotide species mediate cleavage of messenger RNAs and DNA methylation(2,3), respectively, but the biological functions of the 22-nucleotide siRNAs remain unknown. Here we report the identification and characterization of a group of endogenous 22-nucleotide siRNAs that are generated by the DICER-LIKE 2 (DCL2) protein in plants. When cytoplasmic RNA decay and DCL4 are deficient, the resulting massive accumulation of 22-nucleotide siRNAs causes pleiotropic growth disorders, including severe dwarfism, meristem defects and pigmentation. Notably, two genes that encode nitrate reductases-NIA1 and NIA2-produce nearly half of the 22-nucleotide siRNAs. Production of 22-nucleotide siRNAs triggers the amplification of gene silencing and induces translational repression both gene specifically and globally. Moreover, these 22-nucleotide siRNAs preferentially accumulate upon environmental stress, especially those siRNAs derived from NIA1/2, which act to restrain translation, inhibit plant growth and enhance stress responses. Thus, our research uncovers the unique properties of 22-nucleotide siRNAs, and reveals their importance in plant adaptation to environmental stresses.


  
A new approach to molecular biosurveillance of invasive species using DNA metabarcoding 期刊论文
GLOBAL CHANGE BIOLOGY, 2019
作者:  Westfall, Kristen Marie;  Therriault, Thomas W.;  Abbott, Cathryn L.
收藏  |  浏览/下载:7/0  |  提交时间:2020/02/17
ascidian  16S  arthropod  biosurveillance  COI  environmental DNA  genetic marker  invasive species  metabarcoding  mollusk  
The blame game: Using eDNA to identify species-specific tree browsing by red deer (Cervus elaphus) and roe deer (Capreolus capreolus) in a temperate forest 期刊论文
FOREST ECOLOGY AND MANAGEMENT, 2019, 451
作者:  Calkoen, Suzanne T. S. van Beeck;  Leigh-Moy, Kieran;  Cromsigt, Joris P. G. M.;  Spong, Goran;  Lebeau, Leo C.;  Heurich, Marco
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27
Environmental DNA  Foraging patterns  Browsing patterns  Wildlife management  Deer browsing  
GenBank is a reliable resource for 21st century biodiversity research 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (45) : 22651-22656
作者:  Matthieu Leray;  Nancy Knowlton;  Shian-Lei Ho;  Bryan N. Nguyen;  and Ryuji J. Machida
收藏  |  浏览/下载:8/0  |  提交时间:2019/11/27
environmental DNA  metabarcoding  taxonomic assignments  
Precision wildlife medicine: applications of the human-centred precision medicine revolution to species conservation 期刊论文
GLOBAL CHANGE BIOLOGY, 2017, 23 (5)
作者:  Whilde, Jenny;  Martindale, Mark Q.;  Duffy, David J.
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
chytridiomycosis  EcoHealth  emerging disease  environmental DNA (eDNA)  epizootic disease  fibropapillomatosis  One Health  systems medicine  wildlife cancer  wildlife disease