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Peta–electron volt gamma-ray emission from the Crab Nebula 期刊论文
Science, 2021
作者:  The LHAASO Collaboration*†;  Zhen Cao;  F. Aharonian;  Q. An;  Axikegu;  L. X. Bai;  Y. X. Bai;  Y. W. Bao;  D. Bastieri;  X. J. Bi;  Y. J. Bi;  H. Cai;  J. T. Cai;  Zhe Cao;  J. Chang;  J. F. Chang;  B. M. Chen;  E. S. Chen;  J. Chen;  Liang Chen;  Liang Chen;  Long Chen;  M. J. Chen;  M. L. Chen;  Q. H. Chen;  S. H. Chen;  S. Z. Chen;  T. L. Chen;  X. L. Chen;  Y. Chen;  N. Cheng;  Y. D. Cheng;  S. W. Cui;  X. H. Cui;  Y. D. Cui;  B. D’Ettorre Piazzoli;  B. Z. Dai;  H. L. Dai;  Z. G. Dai;  Danzengluobu;  D. della Volpe;  X. J. Dong;  K. K. Duan;  J. H. Fan;  Y. Z. Fan;  Z. X. Fan;  J. Fang;  K. Fang;  C. F. Feng;  L. Feng;  S. H. Feng;  Y. L. Feng;  B. Gao;  C. D. Gao;  L. Q. Gao;  Q. Gao;  W. Gao;  M. M. Ge;  L. S. Geng;  G. H. Gong;  Q. B. Gou;  M. H. Gu;  F. L. Guo;  J. G. Guo;  X. L. Guo;  Y. Q. Guo;  Y. Y. Guo;  Y. A. Han;  H. H. He;  H. N. He;  J. C. He;  S. L. He;  X. B. He;  Y. He;  M. Heller;  Y. K. Hor;  C. Hou;  X. Hou;  H. B. Hu;  S. Hu;  S. C. Hu;  X. J. Hu;  D. H. Huang;  Q. L. Huang;  W. H. Huang;  X. T. Huang;  X. Y. Huang;  Z. C. Huang;  F. Ji;  X. L. Ji;  H. Y. Jia;  K. Jiang;  Z. J. Jiang;  C. Jin;  T. Ke;  D. Kuleshov;  K. Levochkin;  B. B. Li;  Cheng Li;  Cong Li;  F. Li;  H. B. Li;  H. C. Li;  H. Y. Li;  Jian Li;  Jie Li;  K. Li;  W. L. Li;  X. R. Li;  Xin Li;  Xin Li;  Y. Li;  Y. Z. Li;  Zhe Li;  Zhuo Li;  E. W. Liang;  Y. F. Liang;  S. J. Lin;  B. Liu;  C. Liu;  D. Liu;  H. Liu;  H. D. Liu;  J. Liu;  J. L. Liu;  J. S. Liu;  J. Y. Liu;  M. Y. Liu;  R. Y. Liu;  S. M. Liu;  W. Liu;  Y. Liu;  Y. N. Liu;  Z. X. Liu;  W. J. Long;  R. Lu;  H. K. Lv;  B. Q. Ma;  L. L. Ma;  X. H. Ma;  J. R. Mao;  A. Masood;  Z. Min;  W. Mitthumsiri;  T. Montaruli;  Y. C. Nan;  B. Y. Pang;  P. Pattarakijwanich;  Z. Y. Pei;  M. Y. Qi;  Y. Q. Qi;  B. Q. Qiao;  J. J. Qin;  D. Ruffolo;  V. Rulev;  A. Saiz;  L. Shao;  O. Shchegolev;  X. D. Sheng;  J. Y. Shi;  H. C. Song;  Yu. V. Stenkin;  V. Stepanov;  Y. Su;  Q. N. Sun;  X. N. Sun;  Z. B. Sun;  P. H. T. Tam;  Z. B. Tang;  W. W. Tian;  B. D. Wang;  C. Wang;  H. Wang;  H. G. Wang;  J. C. Wang;  J. S. Wang;  L. P. Wang;  L. Y. Wang;  R. N. Wang;  Wei Wang;  Wei Wang;  X. G. Wang;  X. J. Wang;  X. Y. Wang;  Y. Wang;  Y. D. Wang;  Y. J. Wang;  Y. P. Wang;  Z. H. Wang;  Z. X. Wang;  Zhen Wang;  Zheng Wang;  D. M. Wei;  J. J. Wei;  Y. J. Wei;  T. Wen;  C. Y. Wu;  H. R. Wu;  S. Wu;  W. X. Wu;  X. F. Wu;  S. Q. Xi;  J. Xia;  J. J. Xia;  G. M. Xiang;  D. X. Xiao;  G. Xiao;  H. B. Xiao;  G. G. Xin;  Y. L. Xin;  Y. Xing;  D. L. Xu;  R. X. Xu;  L. Xue;  D. H. Yan;  J. Z. Yan;  C. W. Yang;  F. F. Yang;  J. Y. Yang;  L. L. Yang;  M. J. Yang;  R. Z. Yang;  S. B. Yang;  Y. H. Yao;  Z. G. Yao;  Y. M. Ye;  L. Q. Yin;  N. Yin;  X. H. You;  Z. Y. You;  Y. H. Yu;  Q. Yuan;  H. D. Zeng;  T. X. Zeng;  W. Zeng;  Z. K. Zeng;  M. Zha;  X. X. Zhai;  B. B. Zhang;  H. M. Zhang;  H. Y. Zhang;  J. L. Zhang;  J. W. Zhang;  L. X. Zhang;  Li Zhang;  Lu Zhang;  P. F. Zhang;  P. P. Zhang;  R. Zhang;  S. R. Zhang;  S. S. Zhang;  X. Zhang;  X. P. Zhang;  Y. F. Zhang;  Y. L. Zhang;  Yi Zhang;  Yong Zhang;  B. Zhao;  J. Zhao;  L. Zhao;  L. Z. Zhao;  S. P. Zhao;  F. Zheng;  Y. Zheng;  B. Zhou;  H. Zhou;  J. N. Zhou;  P. Zhou;  R. Zhou;  X. X. Zhou;  C. G. Zhu;  F. R. Zhu;  H. Zhu;  K. J. Zhu;  X. Zuo
收藏  |  浏览/下载:14/0  |  提交时间:2021/07/27
Revealing x-ray and gamma ray temporal and spectral similarities in the GRB 190829A afterglow 期刊论文
Science, 2021
作者:  H.E.S.S. Collaboration;  H. Abdalla;  F. Aharonian;  F. Ait Benkhali;  E. O. Angüner;  C. Arcaro;  C. Armand;  T. Armstrong;  H. Ashkar;  M. Backes;  V. Baghmanyan;  V. Barbosa Martins;  A. Barnacka;  M. Barnard;  Y. Becherini;  D. Berge;  K. Bernlöhr;  B. Bi;  E. Bissaldi;  M. Böttcher;  C. Boisson;  J. Bolmont;  M. de Bony de Lavergne;  M. Breuhaus;  F. Brun;  P. Brun;  M. Bryan;  M. Büchele;  T. Bulik;  T. Bylund;  S. Caroff;  A. Carosi;  S. Casanova;  T. Chand;  S. Chandra;  A. Chen;  G. Cotter;  M. Curyło;  J. Damascene Mbarubucyeye;  I. D. Davids;  J. Davies;  C. Deil;  J. Devin;  L. Dirson;  A. Djannati-Ataï;  A. Dmytriiev;  A. Donath;  V. Doroshenko;  L. Dreyer;  C. Duffy;  J. Dyks;  K. Egberts;  F. Eichhorn;  S. Einecke;  G. Emery;  J.-P. Ernenwein;  K. Feijen;  S. Fegan;  A. Fiasson;  G. Fichet de Clairfontaine;  G. Fontaine;  S. Funk;  M. Füßling;  S. Gabici;  Y. A. Gallant;  G. Giavitto;  L. Giunti;  D. Glawion;  J. F. Glicenstein;  M.-H. Grondin;  J. Hahn;  M. Haupt;  G. Hermann;  J. A. Hinton;  W. Hofmann;  C. Hoischen;  T. L. Holch;  M. Holler;  M. Hörbe;  D. Horns;  D. Huber;  M. Jamrozy;  D. Jankowsky;  F. Jankowsky;  A. Jardin-Blicq;  V. Joshi;  I. Jung-Richardt;  E. Kasai;  M. A. Kastendieck;  K. Katarzyński;  U. Katz;  D. Khangulyan;  B. Khélifi;  S. Klepser;  W. Kluźniak;  Nu. Komin;  R. Konno;  K. Kosack;  D. Kostunin;  M. Kreter;  G. Lamanna;  A. Lemière;  M. Lemoine-Goumard;  J.-P. Lenain;  F. Leuschner;  C. Levy;  T. Lohse;  I. Lypova;  J. Mackey;  J. Majumdar;  D. Malyshev;  D. Malyshev;  V. Marandon;  P. Marchegiani;  A. Marcowith;  A. Mares;  G. Martí-Devesa;  R. Marx;  G. Maurin;  P. J. Meintjes;  M. Meyer;  A. Mitchell;  R. Moderski;  L. Mohrmann;  A. Montanari;  C. Moore;  P. Morris;  E. Moulin;  J. Muller;  T. Murach;  K. Nakashima;  A. Nayerhoda;  M. de Naurois;  H. Ndiyavala;  J. Niemiec;  L. Oakes;  P. O’Brien;  H. Odaka;  S. Ohm;  L. Olivera-Nieto;  E. de Ona Wilhelmi;  M. Ostrowski;  S. Panny;  M. Panter;  R. D. Parsons;  G. Peron;  B. Peyaud;  Q. Piel;  S. Pita;  V. Poireau;  A. Priyana Noel;  D. A. Prokhorov;  H. Prokoph;  G. Pühlhofer;  M. Punch;  A. Quirrenbach;  S. Raab;  R. Rauth;  P. Reichherzer;  A. Reimer;  O. Reimer;  Q. Remy;  M. Renaud;  F. Rieger;  L. Rinchiuso;  C. Romoli;  G. Rowell;  B. Rudak;  E. Ruiz-Velasco;  V. Sahakian;  S. Sailer;  H. Salzmann;  D. A. Sanchez;  A. Santangelo;  M. Sasaki;  M. Scalici;  J. Schäfer;  F. Schüssler;  H. M. Schutte;  U. Schwanke;  M. Seglar-Arroyo;  M. Senniappan;  A. S. Seyffert;  N. Shafi;  J. N. S. Shapopi;  K. Shiningayamwe;  R. Simoni;  A. Sinha;  H. Sol;  A. Specovius;  S. Spencer;  M. Spir-Jacob;  Ł. Stawarz;  L. Sun;  R. Steenkamp;  C. Stegmann;  S. Steinmassl;  C. Steppa;  T. Takahashi;  T. Tam;  T. Tavernier;  A. M. Taylor;  R. Terrier;  J. H. E. Thiersen;  D. Tiziani;  M. Tluczykont;  L. Tomankova;  M. Tsirou;  R. Tuffs;  Y. Uchiyama;  D. J. van der Walt;  C. van Eldik;  C. van Rensburg;  B. van Soelen;  G. Vasileiadis;  J. Veh;  C. Venter;  P. Vincent;  J. Vink;  H. J. Völk;  Z. Wadiasingh;  S. J. Wagner;  J. Watson;  F. Werner;  R. White;  A. Wierzcholska;  Yu Wun Wong;  A. Yusafzai;  M. Zacharias;  R. Zanin;  D. Zargaryan;  A. A. Zdziarski;  A. Zech;  S. J. Zhu;  J. Zorn;  S. Zouari;  N. Żywucka;  P. Evans;  K. Page
收藏  |  浏览/下载:33/0  |  提交时间:2021/06/15
COVID‐19 Crisis Reduces Free Tropospheric Ozone across the Northern Hemisphere 期刊论文
Geophysical Research Letters, 2021
作者:  Wolfgang Steinbrecht;  Dagmar Kubistin;  Christian Plass‐;  ;  lmer;  Jonathan Davies;  David W. Tarasick;  Peter von der Gathen;  Holger Deckelmann;  Nis Jepsen;  Rigel Kivi;  Norrie Lyall;  Matthias Palm;  Justus Notholt;  Bogumil Kois;  Peter Oelsner;  Marc Allaart;  Ankie Piters;  Michael Gill;  Roeland Van Malderen;  Andy W. Delcloo;  Ralf Sussmann;  Emmanuel Mahieu;  Christian Servais;  Gonzague Romanens;  Rene Stü;  bi;  Gerard Ancellet;  Sophie Godin‐;  Beekmann;  Shoma Yamanouchi;  Kimberly Strong;  Bryan Johnson;  Patrick Cullis;  Irina Petropavlovskikh;  James W. Hannigan;  Jose‐;  Luis Hernandez;  Ana Diaz Rodriguez;  Tatsumi Nakano;  Fernando Chouza;  Thierry Leblanc;  Carlos Torres;  Omaira Garcia;  Amelie N. Rö;  hling;  Matthias Schneider;  Thomas Blumenstock;  Matt Tully;  Clare Paton‐;  Walsh;  Nicholas Jones;  Richard Querel;  Susan Strahan;  Ryan M. Stauffer;  Anne M. Thompson;  Antje Inness;  Richard Engelen;  Kai‐;  Lan Chang;  Owen R. Cooper
收藏  |  浏览/下载:14/0  |  提交时间:2021/02/17
Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol 期刊论文
Science, 2020
作者:  Lu Zhang;  Yao Zhao;  Yan Gao;  Lijie Wu;  Ruogu Gao;  Qi Zhang;  Yinan Wang;  Chengyao Wu;  Fangyu Wu;  Sudagar S. Gurcha;  Natacha Veerapen;  Sarah M. Batt;  Wei Zhao;  Ling Qin;  Xiuna Yang;  Manfu Wang;  Yan Zhu;  Bing Zhang;  Lijun Bi;  Xian’en Zhang;  Haitao Yang;  Luke W. Guddat;  Wenqing Xu;  Quan Wang;  Jun Li;  Gurdyal S. Besra;  Zihe Rao
收藏  |  浏览/下载:10/0  |  提交时间:2020/06/16
Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform 期刊论文
NATURE, 2020
作者:  Touat, Mehdi;  Li, Yvonne Y.;  Boynton, Adam N.;  Spurr, Liam F.;  Iorgulescu, J. Bryan;  Bohrson, Craig L.;  Cortes-Ciriano, Isidro;  Birzu, Cristina;  Geduldig, Jack E.;  Pelton, Kristine;  Lim-Fat, Mary Jane;  Pal, Sangita;  Ferrer-Luna, Ruben;  Ramkissoon, Shakti H.;  Dubois, Frank;  Bellamy, Charlotte;  Currimjee, Naomi;  Bonardi, Juliana;  Qian Kenin;  Ho, Patricia;  Malinowski, Seth;  Taquet, Leon;  Jones, Robert E.;  Shetty, Aniket;  Chow, Kin-Hoe;  Sharaf, Radwa;  Pavlick, Dean;  Albacker, Lee A.;  Younan, Nadia;  Baldini, Capucine;  Verreault, Maite;  Giry, Marine;  Guillerm, Erell;  Ammari, Samy;  Beuvon, Frederic;  Mokhtari, Karima;  Alentorn, Agusti;  Dehais, Caroline;  Houillier, Caroline;  Laigle-Donadey, Florence;  Psimaras, Dimitri;  Lee, Eudocia Q.;  Nayak, Lakshmi;  McFaline-Figueroa, J. Ricardo;  Carpentier, Alexandre;  Cornu, Philippe;  Capelle, Laurent;  Mathon, Bertrand;  Barnholtz-Sloan, Jill S.;  Chakravarti, Arnab;  Bi, Wenya Linda;  Chiocca, E. Antonio;  Fehnel, Katie Pricola;  Alexandrescu, Sanda;  Chi, Susan N.;  Haas-Kogan, Daphne;  Batchelor, Tracy T.;  Frampton, Garrett M.;  Alexander, Brian M.;  Huang, Raymond Y.;  Ligon, Azra H.;  Coulet, Florence;  Delattre, Jean-Yves;  Hoang-Xuan, Khe;  Meredith, David M.;  Santagata, Sandro;  Duval, Alex;  Sanson, Marc;  Cherniack, Andrew D.;  Wen, Patrick Y.;  Reardon, David A.;  Marabelle, Aurelien;  Park, Peter J.;  Idbaih, Ahmed;  Beroukhim, Rameen;  Bandopadhayay, Pratiti;  Bielle, Franck;  Ligon, Keith L.
收藏  |  浏览/下载:11/0  |  提交时间:2020/07/03

Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate inEscherichia coliowing to the size and occasional instability of the genome(1-3). Therefore, an alternative rapid and robust reverse-genetics platform for RNA viruses would benefit the research community. Here we show the full functionality of a yeast-based synthetic genomics platform to genetically reconstruct diverse RNA viruses, including members of theCoronaviridae,FlaviviridaeandPneumoviridaefamilies. Viral subgenomic fragments were generated using viral isolates, cloned viral DNA, clinical samples or synthetic DNA, and these fragments were then reassembled in one step inSaccharomyces cerevisiaeusing transformation-associated recombination cloning to maintain the genome as a yeast artificial chromosome. T7 RNA polymerase was then used to generate infectious RNA to rescue viable virus. Using this platform, we were able to engineer and generate chemically synthesized clones of the virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(4), which has caused the recent pandemic of coronavirus disease (COVID-19), in only a week after receipt of the synthetic DNA fragments. The technical advance that we describe here facilitates rapid responses to emerging viruses as it enables the real-time generation and functional characterization of evolving RNA virus variants during an outbreak.


A yeast-based synthetic genomics platform is used to reconstruct and characterize large RNA viruses from synthetic DNA fragments  this technique will facilitate the rapid analysis of RNA viruses, such as SARS-CoV-2, during an outbreak.


  
Selective loading and processing of prespacers for precise CRISPR adaptation 期刊论文
NATURE, 2020
作者:  Liu, Guoxia;  Papa, Arianne;  Katchman, Alexander N.;  Zakharov, Sergey I.;  Roybal, Daniel;  Hennessey, Jessica A.;  Kushner, Jared;  Yang, Lin;  Chen, Bi-Xing;  Kushnir, Alexander;  Dangas, Katerina;  Gygi, Steven P.;  Pitt, Geoffrey S.;  Colecraft, Henry M.;  Ben-Johny, Manu;  Kalocsay, Marian;  Marx, Steven O.
收藏  |  浏览/下载:5/0  |  提交时间:2020/07/03

CRISPR-Cas immunity protects prokaryotes against invading genetic elements(1). It uses the highly conserved Cas1-Cas2 complex to establish inheritable memory (spacers)(2-5). How Cas1-Cas2 acquires spacers from foreign DNA fragments (prespacers) and integrates them into the CRISPR locus in the correct orientation is unclear(6,7). Here, using the high spatiotemporal resolution of single-molecule fluorescence, we show that Cas1-Cas2 selects precursors of prespacers from DNA in various forms-including single-stranded DNA and partial duplexes-in a manner that depends on both the length of the DNA strand and the presence of a protospacer adjacent motif (PAM) sequence. We also identify DnaQ exonucleases as enzymes that process the Cas1-Cas2-loaded prespacer precursors into mature prespacers of a suitable size for integration. Cas1-Cas2 protects the PAM sequence from maturation, which results in the production of asymmetrically trimmed prespacers and the subsequent integration of spacers in the correct orientation. Our results demonstrate the kinetic coordination of prespacer precursor selection and PAM trimming, providing insight into the mechanisms that underlie the integration of functional spacers in the CRISPR loci.


Cas1-Cas2 selects precursor prespacers from DNA fragments in a length- and PAM-sequence-dependent manner, and these precursors are trimmed by DnaQ exonucleases to enable integration into the CRISPR locus in the correct orientation.


  
Structural basis of ligand recognition and self-activation of orphan GPR52 期刊论文
NATURE, 2020
作者:  Liu, Guoxia;  Papa, Arianne;  Katchman, Alexander N.;  Zakharov, Sergey I.;  Roybal, Daniel;  Hennessey, Jessica A.;  Kushner, Jared;  Yang, Lin;  Chen, Bi-Xing;  Kushnir, Alexander;  Dangas, Katerina;  Gygi, Steven P.;  Pitt, Geoffrey S.;  Colecraft, Henry M.;  Ben-Johny, Manu;  Kalocsay, Marian;  Marx, Steven O.
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Structures of the orphan G-protein-coupled receptor GPR52 in ligand-free, G-protein-coupled and ligand-bound states reveal that extracellular loop 2 occupies the orthosteric binding pocket and functions as a built-in agonist to activate the receptor.


GPR52 is a class-A orphan G-protein-coupled receptor that is highly expressed in the brain and represents a promising therapeutic target for the treatment of Huntington'  s disease and several psychiatric disorders(1,2). Pathological malfunction of GPR52 signalling occurs primarily through the heterotrimeric G(s) protein(2), but it is unclear how GPR52 and G(s) couple for signal transduction and whether a native ligand or other activating input is required. Here we present the high-resolution structures of human GPR52 in three states: a ligand-free state, a G(s)-coupled self-activation state and a potential allosteric ligand-bound state. Together, our structures reveal that extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, conferring an intrinsically high level of basal activity to GPR52(3). A fully active state is achieved when G(s) is coupled to GPR52 in the absence of an external agonist. The receptor also features a side pocket for ligand binding. These insights into the structure and function of GPR52 could improve our understanding of other self-activated GPCRs, enable the identification of endogenous and tool ligands, and guide drug discovery efforts that target GPR52.


  
Vortex-Wide Detection of Large Aspherical NAT Particles in the Arctic Winter 2011/12 Stratosphere 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2019, 46 (22) : 13420-13429
作者:  Woiwode, W.;  Hoepfner, M.;  Bi, L.;  Khosrawi, F.;  Santee, M. L.
收藏  |  浏览/下载:5/0  |  提交时间:2020/02/17
polar winter  stratosphere  denitrification  nitric acid trihydrate  infrared limb sounding  
Comparison of multiple PM2.5 exposure products for estimating health benefits of emission controls over New York State, USA 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2019, 14 (8)
作者:  Jin, Xiaomeng;  Fiore, Arlene M.;  Civerolo, Kevin;  Bi, Jianzhao;  Liu, Yang;  van Donkelaar, Aaron;  Martin, Randall V.;  Al-Hamdan, Mohammad;  Zhang, Yuqiang;  Insaf, Tabassum Z.;  Kioumourtzoglou, Marianthi-Anna;  He, Mike Z.;  Kinney, Patrick L.
收藏  |  浏览/下载:21/0  |  提交时间:2019/11/27
PM2.5  mortality burden  exposure assessment  remote sensing  
Measurements of Ice Nucleating Particles in Beijing, China 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019, 124 (14) : 8065-8075
作者:  Bi, K.;  McMeeking, G. R.;  Ding, D. P.;  Levin, E. J. T.;  DeMott, P. J.;  Zhao, D. L.;  Wang, F.;  Liu, Q.;  Tian, P.;  Ma, X. C.;  Chen, Y. B.;  Huang, M. Y.;  Zhang, H. L.;  Gordon, T. D.;  Chen, P.
收藏  |  浏览/下载:9/0  |  提交时间:2019/11/27
ice nucleating particle  dust  pollution  Beijing  aerosol  cloud