GSTDTAP

浏览/检索结果: 共80条,第1-10条 帮助

限定条件    
已选(0)清除 条数/页:   排序方式:
Impacts of Two East Asian Atmospheric Circulation Modes on Black Carbon Aerosol Over the Tibetan Plateau in Winter 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2020, 125 (12)
作者:  Yuan, Tiangang;  Chen, Siyu;  Wang, Lin;  Yang, Yaoxian;  Bi, Hongru;  Zhang, Xiaorui;  Zhang, Yue
收藏  |  浏览/下载:13/0  |  提交时间:2020/08/18
black carbon  East Asian winter monsoon  Tibetan Plateau  transport  WRF-Chem  
Source directional apportionment of ambient PM2.5 in urban and industrial sites at a megacity in China 期刊论文
ATMOSPHERIC RESEARCH, 2020, 235
作者:  Liu, Baoshuang;  Li, Yafei;  Wang, Lu;  Bi, Xiaohui;  Dong, Haiyan;  Sun, Xiaoyun;  Xiao, Zhimei;  Zhang, Yufen;  Feng, Yinchang
收藏  |  浏览/下载:14/0  |  提交时间:2020/07/02
Source directional apportionment (SDA)  PM2.5  Backward trajectory analysis  Positive matrix factorization (PMF)  
Vertical characteristics of aerosol hygroscopicity and impacts on optical properties over the North China Plain during winter 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2020, 20 (6) : 3931-3944
作者:  Liu, Quan;  Liu, Dantong;  Gao, Qian;  Tian, Ping;  Wang, Fei;  Zhao, Delong;  Bi, Kai;  Wu, Yangzhou;  Ding, Shuo;  Hu, Kang;  Zhang, Jiale;  Ding, Deping;  Zhao, Chunsheng
收藏  |  浏览/下载:12/0  |  提交时间:2020/07/02
Sources of volatile organic compounds and policy implications for regional ozone pollution control in an urban location of Nanjing, East China 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2020, 20 (6) : 3905-3919
作者:  Zhao, Qiuyue;  Bi, Jun;  Liu, Qian;  Ling, Zhenghao;  Shen, Guofeng;  Chen, Feng;  Qiao, Yuezhen;  Li, Chunyan;  Ma, Zongwei
收藏  |  浏览/下载:8/0  |  提交时间:2020/07/02
Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform 期刊论文
NATURE, 2020
作者:  Touat, Mehdi;  Li, Yvonne Y.;  Boynton, Adam N.;  Spurr, Liam F.;  Iorgulescu, J. Bryan;  Bohrson, Craig L.;  Cortes-Ciriano, Isidro;  Birzu, Cristina;  Geduldig, Jack E.;  Pelton, Kristine;  Lim-Fat, Mary Jane;  Pal, Sangita;  Ferrer-Luna, Ruben;  Ramkissoon, Shakti H.;  Dubois, Frank;  Bellamy, Charlotte;  Currimjee, Naomi;  Bonardi, Juliana;  Qian Kenin;  Ho, Patricia;  Malinowski, Seth;  Taquet, Leon;  Jones, Robert E.;  Shetty, Aniket;  Chow, Kin-Hoe;  Sharaf, Radwa;  Pavlick, Dean;  Albacker, Lee A.;  Younan, Nadia;  Baldini, Capucine;  Verreault, Maite;  Giry, Marine;  Guillerm, Erell;  Ammari, Samy;  Beuvon, Frederic;  Mokhtari, Karima;  Alentorn, Agusti;  Dehais, Caroline;  Houillier, Caroline;  Laigle-Donadey, Florence;  Psimaras, Dimitri;  Lee, Eudocia Q.;  Nayak, Lakshmi;  McFaline-Figueroa, J. Ricardo;  Carpentier, Alexandre;  Cornu, Philippe;  Capelle, Laurent;  Mathon, Bertrand;  Barnholtz-Sloan, Jill S.;  Chakravarti, Arnab;  Bi, Wenya Linda;  Chiocca, E. Antonio;  Fehnel, Katie Pricola;  Alexandrescu, Sanda;  Chi, Susan N.;  Haas-Kogan, Daphne;  Batchelor, Tracy T.;  Frampton, Garrett M.;  Alexander, Brian M.;  Huang, Raymond Y.;  Ligon, Azra H.;  Coulet, Florence;  Delattre, Jean-Yves;  Hoang-Xuan, Khe;  Meredith, David M.;  Santagata, Sandro;  Duval, Alex;  Sanson, Marc;  Cherniack, Andrew D.;  Wen, Patrick Y.;  Reardon, David A.;  Marabelle, Aurelien;  Park, Peter J.;  Idbaih, Ahmed;  Beroukhim, Rameen;  Bandopadhayay, Pratiti;  Bielle, Franck;  Ligon, Keith L.
收藏  |  浏览/下载:11/0  |  提交时间:2020/07/03

Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate inEscherichia coliowing to the size and occasional instability of the genome(1-3). Therefore, an alternative rapid and robust reverse-genetics platform for RNA viruses would benefit the research community. Here we show the full functionality of a yeast-based synthetic genomics platform to genetically reconstruct diverse RNA viruses, including members of theCoronaviridae,FlaviviridaeandPneumoviridaefamilies. Viral subgenomic fragments were generated using viral isolates, cloned viral DNA, clinical samples or synthetic DNA, and these fragments were then reassembled in one step inSaccharomyces cerevisiaeusing transformation-associated recombination cloning to maintain the genome as a yeast artificial chromosome. T7 RNA polymerase was then used to generate infectious RNA to rescue viable virus. Using this platform, we were able to engineer and generate chemically synthesized clones of the virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(4), which has caused the recent pandemic of coronavirus disease (COVID-19), in only a week after receipt of the synthetic DNA fragments. The technical advance that we describe here facilitates rapid responses to emerging viruses as it enables the real-time generation and functional characterization of evolving RNA virus variants during an outbreak.


A yeast-based synthetic genomics platform is used to reconstruct and characterize large RNA viruses from synthetic DNA fragments  this technique will facilitate the rapid analysis of RNA viruses, such as SARS-CoV-2, during an outbreak.


  
Assessing the role of live poultry trade in community-structured transmission of avian influenza in China 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (11) : 5949-5954
作者:  Yang, Qiqi;  Zhao, Xiang;  Lemey, Philippe;  Suchard, Marc A.;  Bi, Yuhai;  Shi, Weifeng;  Liu, Di;  Qi, Wenbao;  Zhang, Guogang;  Stenseth, Nils Chr.;  Pybus, Oliver G.;  Tian, Huaiyu
收藏  |  浏览/下载:10/0  |  提交时间:2020/05/13
avian influenza  poultry trade  phylogeography  community-structured transmission  
Scattering matrices of mineral dust aerosols: a refinement of the refractive index impact 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2020, 20 (5) : 2865-2876
作者:  Huang, Yifan;  Liu, Chao;  Yao, Bin;  Yin, Yan;  Bi, Lei
收藏  |  浏览/下载:6/0  |  提交时间:2020/07/02
Repeated leak detection and repair surveys reduce methane emissions over scale of years 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2020, 15 (3)
作者:  Ravikumar, Arvind P.;  Roda-Stuart, Daniel;  Liu, Ryan;  Bradley, Alexander;  Bergerson, Joule;  Nie, Yuhao;  Zhang, Siduo;  Bi, Xiaotao;  Brandt, Adam R.
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/02
leak detection and repair  methane emissions  policy effectiveness  emissions reductions  
Contrasting contributions of flows on different time scales to tropical cyclone tracks over the South China Sea 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2020, 15 (3)
作者:  Cao, Xi;  Wu, Renguang;  Bi, Mingyu
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/02
tropical cyclone track  the South China Sea  relative contribution  different time scales  
High secondary formation of nitrogen-containing organics (NOCs) and its possible link to oxidized organics and ammonium 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2020, 20 (3) : 1469-1481
作者:  Zhang, Guohua;  Lian, Xiufeng;  Fu, Yuzhen;  Lin, Qinhao;  Li, Lei;  Song, Wei;  Wang, Zhanyong;  Tang, Mingjin;  Chen, Duohong;  Bi, Xinhui;  Wang, Xinming;  Sheng, Guoying
收藏  |  浏览/下载:11/0  |  提交时间:2020/07/02