GSTDTAP

浏览/检索结果: 共7条,第1-7条 帮助

限定条件        
已选(0)清除 条数/页:   排序方式:
A metabolic pathway for bile acid dehydroxylation by the gut microbiome 期刊论文
NATURE, 2020
作者:  Zhong, Miao;  Tran, Kevin;  Min, Yimeng;  Wang, Chuanhao;  Wang, Ziyun;  Dinh, Cao-Thang;  De Luna, Phil;  Yu, Zongqian;  Rasouli, Armin Sedighian;  Brodersen, Peter;  Sun, Song;  Voznyy, Oleksandr;  Tan, Chih-Shan;  Askerka, Mikhail;  Che, Fanglin;  Liu, Min;  Seifitokaldani, Ali;  Pang, Yuanjie;  Lo, Shen-Chuan;  Ip, Alexander;  Ulissi, Zachary;  Sargent, Edward H.
收藏  |  浏览/下载:14/0  |  提交时间:2020/07/03

The biosynthetic pathway that produces the secondary bile acids DCA and LCA in human gut microbes has been fully characterized, engineered into another bacterial host, and used to confer DCA production in germ-free mice-an important proof-of-principle for the engineering of gut microbial pathways.


The gut microbiota synthesize hundreds of molecules, many of which influence host physiology. Among the most abundant metabolites are the secondary bile acids deoxycholic acid (DCA) and lithocholic acid (LCA), which accumulate at concentrations of around 500 mu M and are known to block the growth ofClostridium difficile(1), promote hepatocellular carcinoma(2)and modulate host metabolism via the G-protein-coupled receptor TGR5 (ref.(3)). More broadly, DCA, LCA and their derivatives are major components of the recirculating pool of bile acids(4)  the size and composition of this pool are a target of therapies for primary biliary cholangitis and nonalcoholic steatohepatitis. Nonetheless, despite the clear impact of DCA and LCA on host physiology, an incomplete knowledge of their biosynthetic genes and a lack of genetic tools to enable modification of their native microbial producers limit our ability to modulate secondary bile acid levels in the host. Here we complete the pathway to DCA and LCA by assigning and characterizing enzymes for each of the steps in its reductive arm, revealing a strategy in which the A-B rings of the steroid core are transiently converted into an electron acceptor for two reductive steps carried out by Fe-S flavoenzymes. Using anaerobic in vitro reconstitution, we establish that a set of six enzymes is necessary and sufficient for the eight-step conversion of cholic acid to DCA. We then engineer the pathway intoClostridium sporogenes, conferring production of DCA and LCA on a nonproducing commensal and demonstrating that a microbiome-derived pathway can be expressed and controlled heterologously. These data establish a complete pathway to two central components of the bile acid pool.


  
Gut microbes tune inflammation and lifespan in a mouse model of amyotrophic lateral sclerosis 期刊论文
NATURE, 2020, 582 (7810) : 34-35
作者:  Mega, Emiliano Rodriguez
收藏  |  浏览/下载:14/0  |  提交时间:2020/07/03

The microbiota modulates amyotrophic lateral sclerosis in an animal model.


There is growing evidence that gut microbes can influence disease. Analysis of a mouse model of the neurodegenerative condition amyotrophic lateral sclerosis offers insight into how gut bacteria might contribute to this illness.


  
Statin drugs might boost healthy gut microbes 期刊论文
NATURE, 2020, 581 (7808) : 263-264
作者:  Maxmen, Amy
收藏  |  浏览/下载:12/0  |  提交时间:2020/07/03

Bacterial changes found in people taking cholesterol-lowering medication.


An analysis of faecal samples reveals that obese people who take cholesterol-lowering statin drugs have a '  healthier'  community of gut microorganisms than would be expected. What are the implications of this surprising finding?


  
THE TRICKSTER MICROBES SHAKING UP THE TREE OF LIFE 期刊论文
NATURE, 2019, 569 (7756) : 322-324
作者:  Watson, Traci
收藏  |  浏览/下载:0/0  |  提交时间:2019/11/27
Sewage microbes make a fuel booster 期刊论文
NATURE, 2018, 555 (7698)
作者:  [unavailable]
收藏  |  浏览/下载:0/0  |  提交时间:2019/11/27
Leaf bacterial diversity mediates plant diversity and ecosystem function relationships 期刊论文
NATURE, 2017, 546 (7656) : 145-+
作者:  Laforest-Lapointe, Isabelle;  Paquette, Alain;  Messier, Christian;  Kembel, Steven W.
收藏  |  浏览/下载:7/0  |  提交时间:2019/04/09
Root microbiota drive direct integration of phosphate stress and immunity 期刊论文
NATURE, 2017, 543 (7646) : 513-+
作者:  Castrillo, Gabriel;  Teixeira, Paulo Jose Pereira Lima;  Paredes, Sur Herrera;  Law, Theresa F.;  de Lorenzo, Laura;  Feltcher, Meghan E.;  Finkel, Omri M.;  Breakfield, Natalie W.;  Mieczkowski, Piotr;  Jones, Corbin D.;  Paz-Ares, Javier;  Dangl, Jeffery L.
收藏  |  浏览/下载:9/0  |  提交时间:2019/04/09