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Mechanical regulation of glycolysis via cytoskeleton architecture 期刊论文
NATURE, 2020, 578 (7796) : 621-+
作者:  Faivre, Emily J.;  McDaniel, Keith F.;  Albert, Daniel H.;  Mantena, Srinivasa R.;  Plotnik, Joshua P.;  Wilcox, Denise;  Zhang, Lu;  Bui, Mai H.;  Sheppard, George S.;  Wang, Le;  Sehgal, Vasudha;  Lin, Xiaoyu;  Huang, Xiaoli;  Lu, Xin;  Uziel, Tamar;  Hessler, Paul;  Lam, Lloyd T.;  Bellin, Richard J.;  Mehta, Gaurav;  Fidanze, Steve;  Pratt, John K.;  Liu, Dachun;  Hasvold, Lisa A.;  Sun, Chaohong;  Panchal, Sanjay C.;  Nicolette, John J.;  Fossey, Stacey L.;  Park, Chang H.;  Longenecker, Kenton;  Bigelow, Lance;  Torrent, Maricel;  Rosenberg, Saul H.;  Kati, Warren M.;  Shen, Yu
收藏  |  浏览/下载:15/0  |  提交时间:2020/07/03

The mechanics of the cellular microenvironment continuously modulates cell functions such as growth, survival, apoptosis, differentiation and morphogenesis via cytoskeletal remodelling and actomyosin contractility(1-3). Although all of these processes consume energy(4,5), it is unknown whether and how cells adapt their metabolic activity to variable mechanical cues. Here we report that the transfer of human bronchial epithelial cells from stiff to soft substrates causes a downregulation of glycolysis via proteasomal degradation of the rate-limiting metabolic enzyme phosphofructokinase (PFK). PFK degradation is triggered by the disassembly of stress fibres, which releases the PFK-targeting E3 ubiquitin ligase tripartite motif (TRIM)-containing protein 21 (TRIM21). Transformed non-small-cell lung cancer cells, which maintain high glycolytic rates regardless of changing environmental mechanics, retain PFK expression by downregulating TRIM21, and by sequestering residual TRIM21 on a stress-fibre subset that is insensitive to substrate stiffness. Our data reveal a mechanism by which glycolysis responds to architectural features of the actomyosin cytoskeleton, thus coupling cell metabolism to the mechanical properties of the surrounding tissue. These processes enable normal cells to tune energy production in variable microenvironments, whereas the resistance of the cytoskeleton in response to mechanical cues enables the persistence of high glycolytic rates in cancer cells despite constant alterations of the tumour tissue.


Glycolysis in normal epithelial cells responds to microenvironmental mechanics via the modulation of actin bundles that sequester the phosphofructokinase-targeting ubiquitin ligase TRIM21, a process superseded by persistent actin bundles in cancer cells.


  
Temperature sensitivity of biomass-specific microbial exo-enzyme activities and CO2 efflux is resistant to change across short- and long-term timescales 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (5) : 1793-1807
作者:  Min, Kyungjin;  Buckeridge, Kate;  Ziegler, Susan E.;  Edwards, Kate A.;  Bagchi, Samik;  Billings, Sharon A.
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/26
activation energy  microbial adaptation  microbial biomass  microbial community structure  microbial resilience  microbial resistance  Q(10)  soil warming  
Hopanoid lipids may facilitate aerobic nitrogen fixation in the ocean 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (37) : 18269-18271
作者:  Cornejo-Castillo, Francisco M.;  Zehr, Jonathan P.
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/27
oxygen diffusion barrier  hopanoid lipids  nitrogen fixation  marine cyanobacteria  
Fire severity effects on soil carbon and nutrients and microbial processes in a Siberian larch forest 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (12) : 5841-5852
作者:  Ludwig, Sarah M.;  Alexander, Heather D.;  Kielland, Knut;  Mann, Paul J.;  Natali, Susan M.;  Ruess, Roger W.
收藏  |  浏览/下载:5/0  |  提交时间:2019/04/09
arctic  boreal forest  carbon cycling  climate change  extracellular enzyme activity  fire  permafrost  
Feasting on terrestrial organic matter: Dining in a dark lake changes microbial decomposition 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (11) : 5110-5122
作者:  Fitch, Amelia;  Orland, Chloe;  Willer, David;  Emilson, Erik J. S.;  Tanentzap, Andrew J.
收藏  |  浏览/下载:9/0  |  提交时间:2019/04/09
bacterial production  boreal  carbon cycling  enzyme activity  lake sediments  photo-oxidation  
Differential responses of carbon-degrading enzyme activities to warming: Implications for soil respiration 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (10) : 4816-4826
作者:  Chen, Ji;  Luo, Yiqi;  Garcia-Palacios, Pablo;  Cao, Junji;  Dacal, Marina;  Zhou, Xuhui;  Li, Jianwei;  Xia, Jianyang;  Niu, Shuli;  Yang, Huiyi;  Shelton, Shelby;  Guo, Wei;  van Groenigen, Kees Jan
收藏  |  浏览/下载:11/0  |  提交时间:2019/04/09
cellulase activity  decomposition  extracellular enzyme activity  global warming  ligninase activity  recalcitrant carbon pool  soil microorganisms  soil respiration  
Microbial mechanisms of carbon priming effects revealed during the interaction of crop residue and nutrient inputs in contrasting soils 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (7) : 2775-2790
作者:  Fang, Yunying;  Nazaries, Loic;  Singh, Brajesh K.;  Singh, Bhupinder Pal
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
C-13 isotope  copiotrophs  enzymatic stoichiometry  extracellular enzyme activity  gene abundance  oligotrophs  
Temperature sensitivities of extracellular enzyme V-max and K-m across thermal environments 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (7) : 2884-2897
作者:  Allison, Steven D.;  Romero-Olivares, Adriana L.;  Lu, Ying;  Taylor, John W.;  Treseder, Kathleen K.
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
climate change  fungi  K  (m)  soil extracellular enzyme  temperature sensitivity  thermal adaptation  transition state theory  V  (max)  
Interactions among plants, bacteria, and fungi reduce extracellular enzyme activities under long-term N fertilization 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (6) : 2721-2734
作者:  Carrara, Joseph E.;  Walter, Christopher A.;  Hawkins, Jennifer S.;  Peterjohn, William T.;  Averill, Colin;  Brzostek, Edward R.
收藏  |  浏览/下载:10/0  |  提交时间:2019/04/09
arbuscular mycorrhizal fungi  belowground carbon allocation  extracellular enzymes  microbial community  nitrogen fertilization  plant-microbial interactions  
Structures of beta-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling 期刊论文
NATURE, 2018, 553 (7689) : 501-+
作者:  Lee, Sangwon;  Choi, Jungyuen;  Mohanty, Jyotidarsini;  Sousa, Leiliane P.;  Tome, Francisco;  Pardon, Els;  Steyaert, Jan;  Lemmon, Mark A.;  Lax, Irit;  Schlessinger, Joseph
收藏  |  浏览/下载:5/0  |  提交时间:2019/11/27