Integrative molecular and microanalytical studies of syntrophic partnerships linking C, S, and N cycles in anoxic environments

doi:10.2172/1275739

GSTDTAP > 地球科学

DOI	10.2172/1275739
报告编号	DOE--Caltech-0003940
来源ID	OSTI ID: 1275739
	Integrative molecular and microanalytical studies of syntrophic partnerships linking C, S, and N cycles in anoxic environments
	Orphan, Victoria
	2016-07-15
出版年	2016
页数	6
语种	英语
国家	美国
领域	地球科学
英文摘要	Syntrophy and other forms of symbiotic associations between microorganisms are central to carbon and nutrient cycling in the environment. However, the inherent interdependence of these interactions, dynamic behavior, and frequent existence at thermodynamic limits has hindered our ability to both recognize syntrophic partnerships in nature and effectively study their behavior in the laboratory. To characterize and understand the underlying factors influencing syntrophic associations within complex communities requires a suite of tools that extend beyond basic molecular identification and cultivation. This specifically includes methods that preserve the natural spatial relationships between metabolically interdependent microorganisms while allowing downstream geochemical and/or molecular analysis. With support from this award, we have developed and applied new combinations of molecular, microscopy, and stable isotope-based methodologies that enable the characterization of fundamental links between phylogenetically-identified microorganisms and their specific metabolic activities and interactions in the environment. Through the coupling of fluorescence in situ hybridization (FISH) with cell capture and targeted metagenomics (Magneto-FISH), and FISH + secondary ion mass spectrometry (i.e. FISH-SIMS or FISH-nanoSIMS), we have defined new microbial interactions and the ecophysiology of anaerobic microorganisms involved in environmental methane cycling.
英文关键词	stable isotope probing nanoSIMS anaerobic oxidation of methane fluorescence in situ hybridization archaea
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来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/7277
专题	地球科学
推荐引用方式 GB/T 7714	Orphan, Victoria. Integrative molecular and microanalytical studies of syntrophic partnerships linking C, S, and N cycles in anoxic environments,2016.