Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.2172/1167142 |
报告编号 | DOE-UTK--0004796 |
来源ID | OSTI ID: 1167142 |
MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools | |
Loeffler, Frank E. | |
2014-12-31 | |
出版年 | 2014 |
页数 | 10 |
语种 | 英语 |
国家 | 美国 |
领域 | 地球科学 |
英文摘要 | Uranium (U) contamination in the subsurface is a major remediation challenge at many DOE sites. Traditional site remedies present enormous costs to DOE; hence, enhanced bioremediation technologies (i.e., biostimulation and bioaugmentation) combined with monitoring efforts are being considered as cost-effective corrective actions to address subsurface contamination. This research effort improved understanding of the microbial U reduction process and developed new tools for monitoring microbial activities. Application of these tools will promote science-based site management decisions that achieve contaminant detoxification, plume control, and long-term stewardship in the most efficient manner. The overarching hypothesis was that the design, validation and application of a suite of new molecular and biogeochemical tools advance process understanding, and improve environmental monitoring regimes to assess and predict in situ U immobilization. Accomplishments: This project (i) advanced nucleic acid-based approaches to elucidate the presence, abundance, dynamics, spatial distribution, and activity of metal- and radionuclide-detoxifying bacteria; (ii) developed proteomics workflows for detection of metal reduction biomarker proteins in laboratory cultures and contaminated site groundwater; (iii) developed and demonstrated the utility of U isotopic fractionation using high precision mass spectrometry to quantify U(VI) reduction for a range of reduction mechanisms and environmental conditions; and (iv) validated the new tools using field samples from U-contaminated IFRC sites, and demonstrated their prognostic and diagnostic capabilities in guiding decision making for environmental remediation and long-term site stewardship. |
英文关键词 | Uranium microbial activity immobilization monitoring tools |
URL | 查看原文 |
来源平台 | US Department of Energy (DOE) |
引用统计 | |
文献类型 | 科技报告 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/6632 |
专题 | 地球科学 |
推荐引用方式 GB/T 7714 | Loeffler, Frank E.. MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools,2014. |
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