Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report

doi:10.2172/1077983

GSTDTAP > 地球科学

DOI	10.2172/1077983
报告编号	DOE/ER--63442
来源ID	OSTI ID: 1077983
	Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report
	Cornforth, Michael N. [The University of Texas Medical Branch at Galveston, TX (United States)]
	2013-05-03
出版年	2013
页数	9
语种	英语
国家	美国
领域	地球科学
英文摘要	Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. The aims of this work apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. These aims are: to analyze by multi-flour fluorescence in situ hybridization (mFISH) the chromosomes in clonal descendents of individual human fibroblasts that were previously irradiated; to examine irradiated clones from Aim 1 for submicroscopic deletions by subjecting their DNA to comparative genomic hybridization (CGH) microarray analysis; and to flow-sort aberrant chromosomes from clones containing stable radiation-induced translocations and map the breakpoints to within an average resolution of 100 kb using the technique of 'array painting'.
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来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/6191
专题	地球科学
推荐引用方式 GB/T 7714	Cornforth, Michael N. [The University of Texas Medical Branch at Galveston, TX . Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report,2013.