Defining How a Microbial Cell Senses and Responds to a Redox Active Environment

doi:10.2172/1043690

GSTDTAP > 地球科学

DOI	10.2172/1043690
报告编号	DOE/ER/63427-2 Final Report
来源ID	OSTI ID: 1043690
	Defining How a Microbial Cell Senses and Responds to a Redox Active Environment
	Kenneth H. Nealson
	2012-06-22
出版年	2012
语种	英语
国家	美国
领域	地球科学
英文摘要	This grant was for four years, and the work was designed to look at the mechanisms of extracellular electron transfer by the dissimilatory iron reducing bacteria Shewanella oneidensis MR-1, and other closely related Shewanella strains and species. During this work, we defined many of the basic physiological and biochemical properties of the Shewanella group, Much of which was summarized in review articles. We also finished and published the genome sequence of strain MR-1, the first of the shewanellae to have its genome sequenced. Control at the transcriptional and translational level was studied in collaboration with colleagues at PNNL and ANL. We utilized synchrotron X-ray radiation to image both the bacteria and the metal oxide particles via a technique called STXM, synchrotron X-ray absorption (ref. No.9), and X-ray microbeam analysis. We purified several of the cytochromes involved with metal reduction, and improved gene annotation of the MR-1 genome. The conductive appendages (nanowires) of MR-1 were described and characterized. Comparative genomics and biochemistry revealed that the pathway for the utilization of N-acetyl glucosamine in the various strains of Shewanella exhibited great variability, and had a number of previously unknown genes.
英文关键词	Shawanella electron transport environmental microbiology metal reduction microbial genomics
URL	查看原文
来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/5634
专题	地球科学
推荐引用方式 GB/T 7714	Kenneth H. Nealson. Defining How a Microbial Cell Senses and Responds to a Redox Active Environment,2012.