Global S&T Development Trend Analysis Platform of Resources and Environment
| DOI | 10.2172/1033739 |
| 报告编号 | LLNL-TR-516491 |
| 来源ID | OSTI ID: 1033739 |
| Report for the NGFA-5 project. | |
| West, W. J.; Lucas, J. G.; Gano, K. A. | |
| 2011-11-15 | |
| 出版年 | 2011 |
| 页数 | 108 |
| 语种 | 英语 |
| 国家 | 美国 |
| 领域 | 地球科学 |
| 英文摘要 | The objective of this project is to provide DHS a comprehensive evaluation of the current genomic technologies including genotyping, TaqMan PCR, multiple locus variable tandem repeat analysis (MLVA), microarray and high-throughput DNA sequencing in the analysis of biothreat agents from complex environmental samples. To effectively compare the sensitivity and specificity of the different genomic technologies, we used SNP TaqMan PCR, MLVA, microarray and high-throughput illumine and 454 sequencing to test various strains from B. anthracis, B. thuringiensis, BioWatch aerosol filter extracts or soil samples that were spiked with B. anthracis, and samples that were previously collected during DHS and EPA environmental release exercises that were known to contain B. thuringiensis spores. The results of all the samples against the various assays are discussed in this report. |
| URL | 查看原文 |
| 来源平台 | US Department of Energy (DOE) |
| 引用统计 | |
| 文献类型 | 科技报告 |
| 条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/5442 |
| 专题 | 地球科学 |
| 推荐引用方式 GB/T 7714 | West, W. J.,Lucas, J. G.,Gano, K. A.. Report for the NGFA-5 project.,2011. |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论