Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.2172/985735 |
报告编号 | LBNL-3687E-Poster |
来源ID | OSTI ID: 985735 |
Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris | |
Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin; Mukhopadhyay, Aindrila | |
2010-05-17 | |
出版年 | 2010 |
页数 | 1 |
语种 | 英语 |
国家 | 美国 |
领域 | 地球科学 |
英文摘要 | D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip. |
英文关键词 | D. vulgaris Hildenborough sulfate-reducing bacteria DNA-affinity-purification-chip (DAP-chip) |
URL | 查看原文 |
来源平台 | US Department of Energy (DOE) |
引用统计 | |
文献类型 | 科技报告 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/4976 |
专题 | 地球科学 |
推荐引用方式 GB/T 7714 | Rajeev, Lara,Luning, Eric,Dehal, Paramvir,et al. Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris,2010. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论