Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris

doi:10.2172/985735

GSTDTAP > 地球科学

DOI	10.2172/985735
报告编号	LBNL-3687E-Poster
来源ID	OSTI ID: 985735
	Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris
	Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin; Mukhopadhyay, Aindrila
	2010-05-17
出版年	2010
页数	1
语种	英语
国家	美国
领域	地球科学
英文摘要	D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.
英文关键词	D. vulgaris Hildenborough sulfate-reducing bacteria DNA-affinity-purification-chip (DAP-chip)
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来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/4976
专题	地球科学
推荐引用方式 GB/T 7714	Rajeev, Lara,Luning, Eric,Dehal, Paramvir,et al. Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris,2010.