Effects of Nitrate Exposure on the Functional Structure of a Microbial Community in a Uranium-contaminated Aquifer

doi:10.2172/985928

GSTDTAP > 地球科学

DOI	10.2172/985928
报告编号	LBNL-3734E-Poster
来源ID	OSTI ID: 985928
	Effects of Nitrate Exposure on the Functional Structure of a Microbial Community in a Uranium-contaminated Aquifer
	Van Nostrand, Joy; Waldron, P.; Wu, W.; Zhou, B.; Wu, Liyou; Deng, Ye; Carley, J.; He, Zhili; Gu, B.; Luo, J.; Criddle, C.; Jardine, P.; Hazen, Terry; Zhou, Jizhong
	2010-05-17
出版年	2010
页数	1
语种	英语
国家	美国
领域	地球科学
英文摘要	Increasing nitrogen deposition, increasing atmospheric CO2, and decreasing biodiversity are three main environmental changes occurring on a global scale. The BioCON (Biodiversity, CO2, and Nitrogen) ecological experiment site at the University of Minnesota's Cedar Creek Ecosystem Science Reserve started in 1997, to better understand how these changes would affect soil systems. To understand how increasing nitrogen deposition affects the microbial community diversity, heterogeneity, and functional structure impact soil microbial communities, 12 samples were collected from the BioCON plots in which nitrogenous fertilizer was added to simulate the effect of increasing nitrogen deposition and 12 samples from without added fertilizer. DNA from the 24 samples was extracted using a freeze-grind protocol, amplified, labeled with a fluorescent dye, and then hybridized to GeoChip, a functional gene array containing probes for genes involved in N, S and C cycling, metal resistance and organic contaminant degradation. Detrended correspondence analysis (DCA) of all genes detected was performed to analyze microbial community patterns. The first two axes accounted for 23.5percent of the total variation. The samples fell into two major groups: fertilized and non-fertilized, suggesting that nitrogenous fertilizer had a significant impact on soil microbial community structure and diversity. The functional gene numbers detected in fertilized samples was less that detected in non-fertilizer samples. Functional genes involving in the N cycling were mainly discussed.
英文关键词	BioCON ecological experiment nitrogen desposition microbial community
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来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/4861
专题	地球科学
推荐引用方式 GB/T 7714	Van Nostrand, Joy,Waldron, P.,Wu, W.,et al. Effects of Nitrate Exposure on the Functional Structure of a Microbial Community in a Uranium-contaminated Aquifer,2010.