Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site

doi:10.2172/1010281

GSTDTAP > 地球科学

DOI	10.2172/1010281
报告编号	45234
来源ID	OSTI ID: 1010281
	Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site
	Moser, Duane P.; Bruckner, Jim; Fisher, Jen; Czerwinski, Ken; Russell, Charles E.; Zavarin, Mavrik
	2010-09-01
出版年	2010
语种	英语
国家	美国
领域	地球科学
英文摘要	This U.S. Department of Energy (DOE) Environmental Remediation Sciences Project (ERSP) was designed to test fundamental hypotheses concerning the existence and nature of indigenous microbial populations of Nevada Test Site subsurface nuclear test/detonation cavities. Now called Subsurface Biogeochemical Research (SBR), this programâs Exploratory Research (ER) element, which funded this research, is designed to support high risk, high potential reward projects. Here, five cavities (GASCON, CHANCELLOR, NASH, ALEMAN, and ALMENDRO) and one tunnel (U12N) were sampled using bailers or pumps. Molecular and cultivation-based techniques revealed bacterial signatures at five sites (CHANCELLOR may be lifeless). SSU rRNA gene libraries contained diverse and divergent microbial sequences affiliated with known metal- and sulfur-cycling microorganisms, organic compound degraders, microorganisms from deep mines, and bacteria involved in selenate reduction and arsenite oxidation. Close relatives of Desulforudis audaxviator, a microorganism thought to subsist in the terrestrial deep subsurface on H2 and SO42- produced by radiochemical reactions, was detected in the tunnel waters. NTS-specific media formulations were used to culture and quantify nitrate-, sulfate-, iron-reducing, fermentative, and methanogenic microorganisms. Given that redox manipulations mediated by microorganisms can impact the mobility of DOE contaminants, our results should have implications for management strategies at this and other DOE sites.
英文关键词	Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site
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来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/4792
专题	地球科学
推荐引用方式 GB/T 7714	Moser, Duane P.,Bruckner, Jim,Fisher, Jen,et al. Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site,2010.