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DOI | 10.1016/j.atmosres.2016.08.022 |
PLA2G16 represents a switch between entry and clearance of Picornaviridae | |
Staring, Jacqueline1; von Castelmur, Eleonore1; Blomen, Vincent A.1; van den Hengel, Lisa G.1; Brockmann, Markus1; Baggen, Jim2; Thibaut, Hendrik Jan2; Nieuwenhuis, Joppe1; Janssen, Hans1; van Kuppeveld, Frank J. M.2; Perrakis, Anastassis1; Carette, Jan E.3; Brummelkamp, Thijn R.1,4,5 | |
2017-01-19 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 541期号:7637页码:412-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands; USA; Austria |
英文摘要 | Picornaviruses are a leading cause of human and veterinary infections that result in various diseases, including polio and the common cold. As archetypical non-enveloped viruses, their biology has been extensively studied(1). Although a range of different cell-surface receptors are bound by different picornaviruses(2-7), it is unclear whether common host factors are needed for them to reach the cytoplasm. Using genome-wide haploid genetic screens, here we identify the lipid-modifying enzyme PLA2G16 (refs 8-11) as a picornavirus host factor that is required for a previously unknown event in the viral life cycle. We find that PLA2G16 functions early during infection, enabling virion-mediated genome delivery into the cytoplasm, but not in any virion-assigned step, such as cell binding, endosomal trafficking or pore formation. To resolve this paradox, we screened for suppressors of the Delta PLA2G16 phenotype and identified a mechanism previously implicated in the clearance of intracellular bacteria(12). The sensor of this mechanism, galectin-8 (encoded by LGALS8), detects permeated endosomes and marks them for autophagic degradation, whereas PLA2G16 facilitates viral genome translocation and prevents clearance. This study uncovers two competing processes triggered by virus entry: activation of a pore-activated clearance pathway and recruitment of a phospholipase to enable genome release. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396128800046 |
WOS关键词 | LASSA VIRUS ENTRY ; PHOSPHOLIPASE A(2) ; MAMMALIAN-CELLS ; ENTEROVIRUS 71 ; 2A PROTEINS ; RECEPTOR ; INFECTION ; AUTOPHAGY ; REQUIRES ; SCREENS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/38051 |
专题 | 地球科学 |
作者单位 | 1.Netherlands Canc Inst, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands; 2.Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Div Virol, Yalelaan 1, NL-3584 CL Utrecht, Netherlands; 3.Stanford Univ, Sch Med, Dept Immunol & Microbiol, 299 Campus Dr, Stanford, CA 94305 USA; 4.Austrian Acad Sci, CeMM Res Ctr Mol Med, A-1090 Vienna, Austria; 5.CGC Nl, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands |
推荐引用方式 GB/T 7714 | Staring, Jacqueline,von Castelmur, Eleonore,Blomen, Vincent A.,et al. PLA2G16 represents a switch between entry and clearance of Picornaviridae[J]. NATURE,2017,541(7637):412-+. |
APA | Staring, Jacqueline.,von Castelmur, Eleonore.,Blomen, Vincent A..,van den Hengel, Lisa G..,Brockmann, Markus.,...&Brummelkamp, Thijn R..(2017).PLA2G16 represents a switch between entry and clearance of Picornaviridae.NATURE,541(7637),412-+. |
MLA | Staring, Jacqueline,et al."PLA2G16 represents a switch between entry and clearance of Picornaviridae".NATURE 541.7637(2017):412-+. |
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