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Lakes affect regional climate by modulating surface albedo, surface energy, and moisture budgets. This is especially important for regions such as Northeast Canada with approximately 10 % of the landmass covered by lakes, wetlands and rivers. From the regional hydrology perspective, interactions between lakes and rivers are important as streamflow patterns can be significantly modified by lake storage, and similarly lake levels can be modified by streamflows. In this study, using a suite of experiments performed with the fifth generation Canadian Regional Climate Model (CRCM5) driven by the European Centre for Medium range Weather Forecasting ERA40 reanalysis data at the lateral boundaries for the 1979-2010 period, lake-river-atmosphere interactions and their impact on the regional climate/hydrology of north-east Canada are assessed. In these CRCM5 simulations, a one-dimensional lake model represents lakes, while the rivers are modeled using a distributed routing scheme, and one of the simulations includes interflow, i.e. lateral flow of water in the soil layers. Comparison of CRCM5 simulations with and without lakes suggests significant differences in winter/summer precipitation and winter temperature for the study region. CRCM5 simulations performed with and without lake-river interactions suggest improved representation of streamflows when lake storage and routing are taken into account. Adding the interflow process leads to increased streamflows during summer and fall seasons for the majority of the rivers, causing modest changes to land-atmosphere interactions via modified soil moisture. The impact of interflow on streamflow, obtained in this study, is comparable to the impact of lake-atmosphere interactions on streamflows. This study clearly demonstrates the need for realistic representation of lake-river interactions in regional climate models for realistic simulation of regional hydrology, particularly streamflows.