Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP10208 |
Use of Dental Defects Associated with Low-Dose di(2-Ethylhexyl)Phthalate as an Early Marker of Exposure to Environmental Toxicants | |
Ai Thu Bui; Sophia Houari; Sophia Loiodice; Dominique Bazin; Jérémy Sadoine; Nicolas Roubier; Elsa Vennat; Thu Thuy Tran; Ariane Berdal; Jean-Marc Ricort; Sakina Mhaouty-Kodja; Sylvie Babajko | |
2022-06-22 | |
发表期刊 | Environmental Health Perspectives |
出版年 | 2022 |
英文摘要 | AbstractBackground:Markers of exposure to environmental toxicants are urgently needed. Tooth enamel, with its unique properties, is able to record certain environmental conditions during its formation. Enamel formation and quality are dependent on hormonal regulation and environmental conditions, including exposure to endocrine disrupting chemicals (EDCs). Among EDCs, phthalates such as di-(2-ethylhexyl) phthalate (DEHP) raise concerns about their contribution to various pathologies, including those of mineralized tissues. Objectives:The effects of exposure to low-doses of DEHP on the continually growing incisors were analyzed in mouse males and females. Methods:Adult male and female C57BL/6J mice were exposed daily to 0.5, 5, and DEHP for 12 wk and their incisors clinically examined. Incisors of males were further analyzed by scanning electron microscopy (SEM), micro X-ray computed tomography (micro-computed tomography; ), and nanoindentation for the enamel, histology and real-time quantitative polymerase chain reaction (RT-qPCR) for the dental epithelium. Results:Clinical macroscopic observations of incisors showed various dose-dependent dental lesions such as opacities, scratches, and enamel breakdown in 30.5% of males (10 of 34 total incisors across three independent experiments), and 15.6% of females (7 of 46 incisors) at the highest dose, among which 18.1% (6 of 34 total incisors across three independent experiments) and 8.9% (4 of 46 incisors), respectively, had broken incisors. SEM showed an altered enamel surface and ultrastructure in DEHP-exposed male mice. Further characterization of the enamel defects in males by showed a lower mineral density than controls, and nanoindentation showed a lower enamel hardness during all stages of enamel mineralization, with more pronounced alterations in the external part of the enamel. A delay in enamel mineralization was shown by several approaches (, histology, and RT-qPCR). Discussion:We conclude that DEHP disrupted enamel development in mice by directly acting on dental cells with higher prevalence and severity in males than in females. The time window of DEHP effects on mouse tooth development led to typical alterations of structural, biochemical, and mechanical properties of enamel comparable to other EDCs, such as bisphenol A. The future characterization of dental defects in humans and animals due to environmental toxicants might be helpful in proposing them as early markers of exposure to such molecules. https://doi.org/10.1289/EHP10208 |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/351468 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Ai Thu Bui,Sophia Houari,Sophia Loiodice,et al. Use of Dental Defects Associated with Low-Dose di(2-Ethylhexyl)Phthalate as an Early Marker of Exposure to Environmental Toxicants[J]. Environmental Health Perspectives,2022. |
APA | Ai Thu Bui.,Sophia Houari.,Sophia Loiodice.,Dominique Bazin.,Jérémy Sadoine.,...&Sylvie Babajko.(2022).Use of Dental Defects Associated with Low-Dose di(2-Ethylhexyl)Phthalate as an Early Marker of Exposure to Environmental Toxicants.Environmental Health Perspectives. |
MLA | Ai Thu Bui,et al."Use of Dental Defects Associated with Low-Dose di(2-Ethylhexyl)Phthalate as an Early Marker of Exposure to Environmental Toxicants".Environmental Health Perspectives (2022). |
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