Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1111/gcb.16068 |
Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation | |
Robert W. Murdoch; Gao Chen; Fadime Kara Murdoch; E. Erin Mack; Manuel I. Villalobos Solis; Robert L. Hettich; Frank E. Lö; ffler | |
2022-01-17 | |
发表期刊 | Global Change Biology |
出版年 | 2022 |
英文摘要 | Anthropogenic activities and natural processes release dichloromethane (DCM, methylene chloride), a toxic chemical with substantial ozone-depleting capacity. Specialized anaerobic bacteria metabolize DCM; however, the genetic basis for this process has remained elusive. Comparative genomics of the three known anaerobic DCM-degrading bacterial species revealed a homologous gene cluster, designated the methylene chloride catabolism (mec) gene cassette, comprising 8–10 genes encoding proteins with 79.6%–99.7% amino acid identities. Functional annotation identified genes encoding a corrinoid-dependent methyltransferase system, and shotgun proteomics applied to two DCM-catabolizing cultures revealed high expression of proteins encoded on the mec gene cluster during anaerobic growth with DCM. In a DCM-contaminated groundwater plume, the abundance of mec genes strongly correlated with DCM concentrations (R2 = 0.71–0.85) indicating their potential value as process-specific bioremediation biomarkers. mec gene clusters were identified in metagenomes representing peat bogs, the deep subsurface, and marine ecosystems including oxygen minimum zones (OMZs), suggesting a capacity for DCM degradation in diverse habitats. The broad distribution of anaerobic DCM catabolic potential infers a role for DCM as an energy source in various environmental systems, and implies that the global DCM flux (i.e., the rate of formation minus the rate of consumption) might be greater than emission measurements suggest. |
领域 | 气候变化 ; 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/346106 |
专题 | 气候变化 资源环境科学 |
推荐引用方式 GB/T 7714 | Robert W. Murdoch,Gao Chen,Fadime Kara Murdoch,et al. Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation[J]. Global Change Biology,2022. |
APA | Robert W. Murdoch.,Gao Chen.,Fadime Kara Murdoch.,E. Erin Mack.,Manuel I. Villalobos Solis.,...&ffler.(2022).Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation.Global Change Biology. |
MLA | Robert W. Murdoch,et al."Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation".Global Change Biology (2022). |
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