Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice

doi:10.1126/science.abi4506

GSTDTAP > 气候变化

DOI	10.1126/science.abi4506
	Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice
	David R. Martinez; Alexandra Schäfer; Sarah R. Leist; Gabriela De la Cruz; Ande West; Elena N. Atochina-Vasserman; Lisa C. Lindesmith; Norbert Pardi; Robert Parks; Maggie Barr; Dapeng Li; Boyd Yount; Kevin O. Saunders; Drew Weissman; Barton F. Haynes; Stephanie A. Montgomery; Ralph S. Baric
	2021-08-27
发表期刊	Science
出版年	2021
英文摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third coronavirus that has emerged as a serious human pathogen in the past 20 years. Treatment strategies that are broadly protective against current and future SARS-like coronaviruses are needed. Martinez et al. took on this challenge by developing vaccines based on chimeras of the viral spike protein. The messenger RNA vaccines encode spike proteins composed of domain modules from epidemic and pandemic coronaviruses, as well as bat coronaviruses with the potential to cross to humans. In aged mice vulnerable to infection, the chimeric vaccines protected against challenge from SARS-CoV, SARS-CoV-2 and tested variants of concern, and zoonotic coronaviruses with pandemic potential. Science , abi4506, this issue p. [991][1] The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 and SARS-CoV-2 in 2019 highlights the need to develop universal vaccination strategies against the broader Sarbecovirus subgenus. Using chimeric spike designs, we demonstrate protection against challenge from SARS-CoV, SARS-CoV-2, SARS-CoV-2 B.1.351, bat CoV (Bt-CoV) RsSHC014, and a heterologous Bt-CoV WIV-1 in vulnerable aged mice. Chimeric spike messenger RNAs (mRNAs) induced high levels of broadly protective neutralizing antibodies against high-risk Sarbecoviruses. By contrast, SARS-CoV-2 mRNA vaccination not only showed a marked reduction in neutralizing titers against heterologous Sarbecoviruses, but SARS-CoV and WIV-1 challenge in mice resulted in breakthrough infections. Chimeric spike mRNA vaccines efficiently neutralized D614G, mink cluster five, and the UK B.1.1.7 and South African B.1.351 variants of concern. Thus, multiplexed-chimeric spikes can prevent SARS-like zoonotic coronavirus infections with pandemic potential. [1]: /lookup/doi/10.1126/science.abi4506
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/336658
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	David R. Martinez,Alexandra Schäfer,Sarah R. Leist,et al. Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice[J]. Science,2021.
APA	David R. Martinez.,Alexandra Schäfer.,Sarah R. Leist.,Gabriela De la Cruz.,Ande West.,...&Ralph S. Baric.(2021).Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice.Science.
MLA	David R. Martinez,et al."Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice".Science (2021).