Maturation of the matrix and viral membrane of HIV-1

doi:10.1126/science.abe6821

GSTDTAP > 气候变化

DOI	10.1126/science.abe6821
	Maturation of the matrix and viral membrane of HIV-1
	Kun Qu; Zunlong Ke; Vojtech Zila; Maria Anders-Össwein; Bärbel Glass; Frauke Mücksch; Rainer Müller; Carsten Schultz; Barbara Müller; Hans-Georg Kräusslich; John A. G. Briggs
	2021-08-06
发表期刊	Science
出版年	2021
英文摘要	Nascent HIV particles assemble at the plasma membrane of an infected cell and bud into a membrane-enveloped, immature virion. Assembly and budding are driven by a polyprotein called Gag, which consists of a matrix domain (MA) that is recruited to the plasma membrane, a capsid domain (CA) responsible for self-assembly, and a nucleocapsid domain (NC) that recruits the viral RNA genome. Gag cleavage results in a structural rearrangement that produces the mature virion. Qu et al. imaged mature and immature HIV particles by electron tomography and focused in on the MA domain (see the Perspective by Hikichi and Freed). They found that MA rearranges between two distinct hexameric lattices, and mature MA modulates the viral membrane by binding to a membrane lipid. This finding suggests that MA may play functional roles in the mature virion. Science , abe6821, this issue p. [700][1]; see also abj9075, p. [621][2] Gag, the primary structural protein of HIV-1, is recruited to the plasma membrane for virus assembly by its matrix (MA) domain. Gag is subsequently cleaved into its component domains, causing structural maturation to repurpose the virion for cell entry. We determined the structure and arrangement of MA within immature and mature HIV-1 through cryo–electron tomography. We found that MA rearranges between two different hexameric lattices upon maturation. In mature HIV-1, a lipid extends out of the membrane to bind with a pocket in MA. Our data suggest that proteolytic maturation of HIV-1 not only assembles the viral capsid surrounding the genome but also repurposes the membrane-bound MA lattice for an entry or postentry function and results in the partial removal of up to 2500 lipids from the viral membrane. [1]: /lookup/doi/10.1126/science.abe6821 [2]: /lookup/doi/10.1126/science.abj9075
领域	气候变化 ; 资源环境
URL	查看原文
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/335584
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Kun Qu,Zunlong Ke,Vojtech Zila,et al. Maturation of the matrix and viral membrane of HIV-1[J]. Science,2021.
APA	Kun Qu.,Zunlong Ke.,Vojtech Zila.,Maria Anders-Össwein.,Bärbel Glass.,...&John A. G. Briggs.(2021).Maturation of the matrix and viral membrane of HIV-1.Science.
MLA	Kun Qu,et al."Maturation of the matrix and viral membrane of HIV-1".Science (2021).