An isoform of Dicer protects mammalian stem cells against multiple RNA viruses

doi:10.1126/science.abg2264

GSTDTAP > 气候变化

DOI	10.1126/science.abg2264
	An isoform of Dicer protects mammalian stem cells against multiple RNA viruses
	Enzo Z. Poirier; Michael D. Buck; Probir Chakravarty; Joana Carvalho; Bruno Frederico; Ana Cardoso; Lyn Healy; Rachel Ulferts; Rupert Beale; Caetano Reis e Sousa
	2021-07-09
发表期刊	Science
出版年	2021
英文摘要	Stem cells have a pivotal role in maintaining tissue architecture, integrity, and renewal. Poirier et al. demonstrate that mammalian stem cells can protect themselves from some RNA viruses by expressing an alternatively spliced isoform of the enzyme Dicer called aviD, which potentiates antiviral RNA interference (see the Perspective by Shahrudin and Ding). aviD acts by cleaving long, base-paired viral RNAs to generate small interfering RNAs that direct the sequence-specific cleavage of homologous viral RNAs. This process is reminiscent of that in insects and worms, which also use Dicer-dependent RNA interference in antiviral defense, and contrasts with mammalian differentiated cells, which generally rely on the interferon system to combat virus infection. Science , abg2264, this issue p. [231][1]; see also abj5673, p. [160][2] In mammals, early resistance to viruses relies on interferons, which protect differentiated cells but not stem cells from viral replication. Many other organisms rely instead on RNA interference (RNAi) mediated by a specialized Dicer protein that cleaves viral double-stranded RNA. Whether RNAi also contributes to mammalian antiviral immunity remains controversial. We identified an isoform of Dicer, named antiviral Dicer (aviD), that protects tissue stem cells from RNA viruses—including Zika virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—by dicing viral double-stranded RNA to orchestrate antiviral RNAi. Our work sheds light on the molecular regulation of antiviral RNAi in mammalian innate immunity, in which different cell-intrinsic antiviral pathways can be tailored to the differentiation status of cells. [1]: /lookup/doi/10.1126/science.abg2264 [2]: /lookup/doi/10.1126/science.abj5673
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/334285
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Enzo Z. Poirier,Michael D. Buck,Probir Chakravarty,et al. An isoform of Dicer protects mammalian stem cells against multiple RNA viruses[J]. Science,2021.
APA	Enzo Z. Poirier.,Michael D. Buck.,Probir Chakravarty.,Joana Carvalho.,Bruno Frederico.,...&Caetano Reis e Sousa.(2021).An isoform of Dicer protects mammalian stem cells against multiple RNA viruses.Science.
MLA	Enzo Z. Poirier,et al."An isoform of Dicer protects mammalian stem cells against multiple RNA viruses".Science (2021).