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It is anticipated that the performance of real-time (RT) GNSS meteorology can be further improved by incorporating observations from multiple Global Navigation Satellite System (GNSS), including GPS, GLONASS, Galileo, and BeiDou. In this paper, an operational RT system for extracting zenith troposphere delay (ZTD) using a modified version of the Precise Point Positioning With Integer and Zero-difference Ambiguity Resolution Demonstrator (PPP-WIZARD) was established. GNSS, including GPS, GLONASS, and Galileo, observation streams were processed using RT Precise Point Positioning (PPP) strategy based on RT satellite orbit/clock products from the Centre National d'Etudes Spatiales. An experiment covering 30 days was conducted, in which the observation streams of 20 globally distributed stations were processed. The initialization time and accuracy of the RT troposphere results using single-system and multisystem observations were evaluated. The effect of PPP ambiguity resolution was also evaluated. Results reveal that RT troposphere estimates based on single-system observations can both be applied in weather nowcasting, in which the GPS-only solution is better than the GLONASS-only solution. The performance can also be improved by PPP ambiguity resolution and utilizing GNSS observations. Specifically, we notice that ambiguity resolution is more effective in improving the accuracy of ZTD, whereas the initialization process can be better accelerated by GNSS observations. Combining all techniques, the RT troposphere results with an average accuracy of about 8 mm in ZTD can be achieved after an initialization process of approximately 8.5 min, which demonstrates superior results for applying GNSS observations and ambiguity resolution for RT meteorological applications.