Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes

doi:10.1126/science.abg5268

GSTDTAP > 气候变化

DOI	10.1126/science.abg5268
	Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes
	William N. Voss; Yixuan J. Hou; Nicole V. Johnson; George Delidakis; Jin Eyun Kim; Kamyab Javanmardi; Andrew P. Horton; Foteini Bartzoka; Chelsea J. Paresi; Yuri Tanno; Chia-Wei Chou; Shawn A. Abbasi; Whitney Pickens; Katia George; Daniel R. Boutz; Dalton M. Towers; Jonathan R. McDaniel; Daniel Billick; Jule Goike; Lori Rowe; Dhwani Batra; Jan Pohl; Justin Lee; Shivaprakash Gangappa; Suryaprakash Sambhara; Michelle Gadush; Nianshuang Wang; Maria D. Person; Brent L. Iverson; Jimmy D. Gollihar; John M. Dye; Andrew S. Herbert; Ilya J. Finkelstein; Ralph S. Baric; Jason S. McLellan; George Georgiou; Jason J. Lavinder; Gregory C. Ippolito
	2021-06-04
发表期刊	Science
出版年	2021
英文摘要	Most analyses of the antibody responses induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have focused on antibodies cloned from memory B cells. This approach has led researchers to conclude that neutralizing antibodies (nAbs) primarily target the receptor-binding domain (RBD) of the virus's spike protein. Voss et al. took a different approach, using proteomic deconvolution of the serum immunoglobulin G antibody repertoire from four COVID-19 convalescent patients. They found that the nAb response was largely directed against epitopes such as the N-terminal domain (NTD), which lie outside the RBD. Several of these nAbs were shared among donors and targeted an NTD epitope that is frequently mutated by variants of concern. Science , abg5268, this issue p. [1108][1] The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an amino (N)-terminal domain (NTD)–directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multidonor class of “public” antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that “public” NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape. [1]: /lookup/doi/10.1126/science.abg5268
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/329880
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	William N. Voss,Yixuan J. Hou,Nicole V. Johnson,et al. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes[J]. Science,2021.
APA	William N. Voss.,Yixuan J. Hou.,Nicole V. Johnson.,George Delidakis.,Jin Eyun Kim.,...&Gregory C. Ippolito.(2021).Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.Science.
MLA	William N. Voss,et al."Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes".Science (2021).