Imaging translation dynamics in live embryos reveals spatial heterogeneities

doi:10.1126/science.abc3483

GSTDTAP > 气候变化

DOI	10.1126/science.abc3483
	Imaging translation dynamics in live embryos reveals spatial heterogeneities
	Jeremy Dufourt; Maelle Bellec; Antonio Trullo; Matthieu Dejean; Sylvain De Rossi; Cyril Favard; Mounia Lagha
	2021-05-21
发表期刊	Science
出版年	2021
英文摘要	During development, precise control of gene expression establishes reproducible patterns, leading to the formation of organs at the right time and place. The emergence of developmental patterns has been primarily studied at the transcriptional level, but the fate of these transcripts has received little attention. Dufourt et al. used the SunTag labeling method to image the dynamics of translation of individual messenger RNA (mRNA) molecules in living fruit fly embryos. This work revealed “translation factories”—clusters of mRNA and translation machinery—and heterogeneities in the efficiency of translation between identical mRNAs. Science , abc3483, this issue p. [840][1] Much is known about the factors involved in the translation of messenger RNA (mRNA) into protein; however, this multistep process has not been imaged in living multicellular organisms. Here, we deploy the SunTag method to visualize and quantify the timing, location, and kinetics of the translation of single mRNAs in living Drosophila embryos. By focusing on the translation of the conserved major epithelial-mesenchymal transition–inducing transcription factor Twist, we identify spatial heterogeneity in mRNA translation efficiency and reveal the existence of translation factories, where clustered mRNAs are cotranslated preferentially at basal perinuclear regions. Observing the location and dynamics of mRNA translation in a living multicellular organism opens avenues for understanding gene regulation during development. [1]: /lookup/doi/10.1126/science.abc3483
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/328827
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jeremy Dufourt,Maelle Bellec,Antonio Trullo,et al. Imaging translation dynamics in live embryos reveals spatial heterogeneities[J]. Science,2021.
APA	Jeremy Dufourt.,Maelle Bellec.,Antonio Trullo.,Matthieu Dejean.,Sylvain De Rossi.,...&Mounia Lagha.(2021).Imaging translation dynamics in live embryos reveals spatial heterogeneities.Science.
MLA	Jeremy Dufourt,et al."Imaging translation dynamics in live embryos reveals spatial heterogeneities".Science (2021).