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DOI | 10.1002/2016JD025254 |
Reconstitution of the tubular endoplasmic reticulum network with purified components | |
Powers, Robert E.1,2; Wang, Songyu1,2; Liu, Tina Y.1,2,3; Rapoport, Tom A.1,2 | |
2017-03-09 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 543期号:7644页码:257-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Organelles display characteristic morphologies that are intimately tied to their cellular function, but how organelles are shaped is poorly understood. The endoplasmic reticulum is particularly intriguing, as it comprises morphologically distinct domains, including a dynamic network of interconnected membrane tubules. Several membrane proteins have been implicated in network formation(1-5), but how exactly they mediate network formation and whether they are all required are unclear. Here we reconstitute a dynamic tubular membrane network with purified endoplasmic reticulum proteins. Proteoliposomes containing the membrane-fusing GTPase Sey1p (refs 6, 7) and the curvature-stabilizing protein Yop1p (refs 8, 9) from Saccharomyces cerevisiae form a tubular network upon addition of GTP. The tubules rapidly fragment when GTP hydrolysis of Sey1p is inhibited, indicating that network maintenance requires continuous membrane fusion and that Yop1p favours the generation of highly curved membrane structures. Sey1p also forms networks with other curvature-stabilizing proteins, including reticulon(8) and receptor expression-enhancing proteins (REEPs)(10) from different species. Atlastin, the vertebrate orthologue of Sey1p(6,11), forms a GTP-hydrolysis-dependent network on its own, serving as both a fusion and curvature-stabilizing protein. Our results show that organelle shape can be generated by a surprisingly small set of proteins and represents an energy-dependent steady state between formation and disassembly. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000395688700042 |
WOS关键词 | HOMOTYPIC ER FUSION ; MEMBRANE-PROTEINS ; AMPHIPATHIC HELIX ; PROVIDE INSIGHT ; ATLASTIN ; GENERATION ; CELLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/32794 |
专题 | 气候变化 |
作者单位 | 1.Harvard Med Sch, Howard Hughes Med Inst, 240 Longwood Ave, Boston, MA 02115 USA; 2.Harvard Med Sch, Dept Cell Biol, 240 Longwood Ave, Boston, MA 02115 USA; 3.Univ Calif Berkeley, 731 Stanley Hall,MS 3220, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Powers, Robert E.,Wang, Songyu,Liu, Tina Y.,et al. Reconstitution of the tubular endoplasmic reticulum network with purified components[J]. NATURE,2017,543(7644):257-+. |
APA | Powers, Robert E.,Wang, Songyu,Liu, Tina Y.,&Rapoport, Tom A..(2017).Reconstitution of the tubular endoplasmic reticulum network with purified components.NATURE,543(7644),257-+. |
MLA | Powers, Robert E.,et al."Reconstitution of the tubular endoplasmic reticulum network with purified components".NATURE 543.7644(2017):257-+. |
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