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Nitrogen is one of the three macronutrients required by plants for growth and development, along with phosphorus and potassium. Nitrogen-rich condition induces plant growth, particularly the growth of stems and leaves, while delaying flowering. On the other hand, in some plants, low-nitrogen conditions lead to a change from growth mode to reproductive mode, therefore accelerating flowering. However, the molecular mechanisms that regulate flowering under these conditions are not known.

A team of scientists led by Associate Professor Takeo Sato of Hokkaido University's Graduate School of Life Science has revealed the molecular mechanism responsible for the acceleration of flowering in Arabidopsis under low nitrogen conditions. Their findings were published in the journal Proceedings of the National Academy of Sciences (PNAS).

Arabidopsis, a cruciferous plant, is well known as a model plant in biology and has an extensive database of its protein expression. In the current study, the team first identified a set of proteins involved in flowering that became active as a result of changes in nitrogen level. One of these was the gene regulation factor FLOWERING BHLH 4 (FBH4). Through experiments using FBH4 deficient plants, this protein was found to be responsible for accelerated flowering under low-nitrogen conditions.

Further investigation suggested that FBH4 is extensively phosphorylated by another protein called SnRK1. Low-nitrogen conditions suppress SnRK1 activity, which in turn results in the dephosphorylation of FBH4. The dephosphorylated FBH4 moves to the nucleus to activate genes responsible for flowering. Dephosphorylated FBH4 is also responsible for controlling the expression of other genes vital for plant survival under low nitrogen conditions, particularly those related to nitrogen recycling and remobilization.

The scientists concluded that, in response to inadequate nitrogen, Arabidopsis plants appear to precisely control gene expression related to developmental and metabolic processes required for flowering through FBH4. "The FBH family of genes is present in major crop plants," says Takeo Sato. "Crop plants exhibit early flowering under low-nitrogen conditions; if we can control FBH activities in these crop plants, it might be an effective way to sustainably increase agricultural production."

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Materials provided by Hokkaido University. Note: Content may be edited for style and length.

Journal Reference:

1. Miho Sanagi, Shoki Aoyama, Akio Kubo, Yu Lu, Yasutake Sato, Shogo Ito, Mitsutomo Abe, Nobutaka Mitsuda, Masaru Ohme-Takagi, Takatoshi Kiba, Hirofumi Nakagami, Filip Rolland, Junji Yamaguchi, Takato Imaizumi, Takeo Sato. Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis. Proceedings of the National Academy of Sciences, 2021; 118 (19): e2022942118 DOI: 10.1073/pnas.2022942118