Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.2023298118 |
Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea | |
Romain Savary; Daniel J. Barshis; Christian R. Voolstra; Anny Cárdenas; Nicolas R. Evensen; Guilhem Banc-Prandi; Maoz Fine; Anders Meibom | |
2021-05-11 | |
发表期刊 | Proceedings of the National Academy of Sciences |
出版年 | 2021 |
英文摘要 | Corals from the northern Red Sea and Gulf of Aqaba exhibit extreme thermal tolerance. To examine the underlying gene expression dynamics, we exposed Stylophora pistillata from the Gulf of Aqaba to short-term (hours) and long-term (weeks) heat stress with peak seawater temperatures ranging from their maximum monthly mean of 27 °C (baseline) to 29.5 °C, 32 °C, and 34.5 °C. Corals were sampled at the end of the heat stress as well as after a recovery period at baseline temperature. Changes in coral host and symbiotic algal gene expression were determined via RNA-sequencing (RNA-Seq). Shifts in coral microbiome composition were detected by complementary DNA (cDNA)-based 16S ribosomal RNA (rRNA) gene sequencing. In all experiments up to 32 °C, RNA-Seq revealed fast and pervasive changes in gene expression, primarily in the coral host, followed by a return to baseline gene expression for the majority of coral (>94%) and algal (>71%) genes during recovery. At 34.5 °C, large differences in gene expression were observed with minimal recovery, high coral mortality, and a microbiome dominated by opportunistic bacteria (including Vibrio species), indicating that a lethal temperature threshold had been crossed. Our results show that the S. pistillata holobiont can mount a rapid and pervasive gene expression response contingent on the amplitude and duration of the thermal stress. We propose that the transcriptomic resilience and transcriptomic acclimation observed are key to the extraordinary thermal tolerance of this holobiont and, by inference, of other northern Red Sea coral holobionts, up to seawater temperatures of at least 32 °C, that is, 5 °C above their current maximum monthly mean. |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/325901 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Romain Savary,Daniel J. Barshis,Christian R. Voolstra,et al. Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea[J]. Proceedings of the National Academy of Sciences,2021. |
APA | Romain Savary.,Daniel J. Barshis.,Christian R. Voolstra.,Anny Cárdenas.,Nicolas R. Evensen.,...&Anders Meibom.(2021).Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea.Proceedings of the National Academy of Sciences. |
MLA | Romain Savary,et al."Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea".Proceedings of the National Academy of Sciences (2021). |
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