Noncanonical DNA polymerization by aminoadenine-based siphoviruses

doi:10.1126/science.abe6542

GSTDTAP > 气候变化

DOI	10.1126/science.abe6542
	Noncanonical DNA polymerization by aminoadenine-based siphoviruses
	Valerie Pezo; Faten Jaziri; Pierre-Yves Bourguignon; Dominique Louis; Deborah Jacobs-Sera; Jef Rozenski; Sylvie Pochet; Piet Herdewijn; Graham F. Hatfull; Pierre-Alexandre Kaminski; Philippe Marliere
	2021-04-30
发表期刊	Science
出版年	2021
英文摘要	Four nucleobases. adenine (A), cytosine (C), guanine (G), and thymine (T), are usually thought to be invariable in DNA. In bacterial viruses, however, each of the DNA bases have variations that help them to escape degradation by bacterial restriction enzymes. In the genome of cyanophage S-2L, A is completely replaced by diaminopurine (Z), which forms three hydrogen bonds with T and thus creates non–Watson-Crick base pairing in the DNA of this virus (see the Perspective by Grome and Isaacs). Zhou et al. and Sleiman et al. determined the biochemical pathway that produces Z, which revealed more Z genomes in viruses hosted in bacteria distributed widely in the environment and phylogeny. Pezo et al. identified a DNA polymerase that incorporates Z into DNA while rejecting A. These findings enrich our understanding of biodiversity and expand the genetic palette for synthetic biology. Science , this issue p. [512][1], [516][2], [520][3]; see also p. [460][4] Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3′-exonuclease but lacks 5′-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution. [1]: /lookup/doi/10.1126/science.abe4882 [2]: /lookup/doi/10.1126/science.abe6494 [3]: /lookup/doi/10.1126/science.abe6542 [4]: /lookup/doi/10.1126/science.abh3571
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/325042
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Valerie Pezo,Faten Jaziri,Pierre-Yves Bourguignon,et al. Noncanonical DNA polymerization by aminoadenine-based siphoviruses[J]. Science,2021.
APA	Valerie Pezo.,Faten Jaziri.,Pierre-Yves Bourguignon.,Dominique Louis.,Deborah Jacobs-Sera.,...&Philippe Marliere.(2021).Noncanonical DNA polymerization by aminoadenine-based siphoviruses.Science.
MLA	Valerie Pezo,et al."Noncanonical DNA polymerization by aminoadenine-based siphoviruses".Science (2021).