Replication timing maintains the global epigenetic state in human cells

doi:10.1126/science.aba5545

GSTDTAP > 气候变化

DOI	10.1126/science.aba5545
	Replication timing maintains the global epigenetic state in human cells
	Kyle N. Klein; Peiyao A. Zhao; Xiaowen Lyu; Takayo Sasaki; Daniel A. Bartlett; Amar M. Singh; Ipek Tasan; Meng Zhang; Lotte P. Watts; Shin-ichiro Hiraga; Toyoaki Natsume; Xuemeng Zhou; Timour Baslan; Danny Leung; Masato T. Kanemaki; Anne D. Donaldson; Huimin Zhao; Stephen Dalton; Victor G. Corces; David M. Gilbert
	2021-04-23
发表期刊	Science
出版年	2021
英文摘要	The temporal order of DNA replication is conserved from yeast to humans, but its biological significance remains unclear. Klein et al. eliminated the protein RIF1, a master regulator of replication timing, in several human cell lines. RIF1 loss during the G1 phase of the cell cycle resulted in a heterogeneous, nearly random replication timing program from the first S phase that persisted even in stable RIF1-null clones. Altered replication timing was followed by replication-dependent redistribution of active and repressive histone modifications and alterations in genome architecture. These results support a model in which replication timing orchestrates the epigenetic state of newly replicated chromatin. Science , this issue p. [371][1] The temporal order of DNA replication [replication timing (RT)] is correlated with chromatin modifications and three-dimensional genome architecture; however, causal links have not been established, largely because of an inability to manipulate the global RT program. We show that loss of RIF1 causes near-complete elimination of the RT program by increasing heterogeneity between individual cells. RT changes are coupled with widespread alterations in chromatin modifications and genome compartmentalization. Conditional depletion of RIF1 causes replication-dependent disruption of histone modifications and alterations in genome architecture. These effects were magnified with successive cycles of altered RT. These results support models in which the timing of chromatin replication and thus assembly plays a key role in maintaining the global epigenetic state. [1]: /lookup/doi/10.1126/science.aba5545
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/324076
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Kyle N. Klein,Peiyao A. Zhao,Xiaowen Lyu,et al. Replication timing maintains the global epigenetic state in human cells[J]. Science,2021.
APA	Kyle N. Klein.,Peiyao A. Zhao.,Xiaowen Lyu.,Takayo Sasaki.,Daniel A. Bartlett.,...&David M. Gilbert.(2021).Replication timing maintains the global epigenetic state in human cells.Science.
MLA	Kyle N. Klein,et al."Replication timing maintains the global epigenetic state in human cells".Science (2021).