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DOI | 10.1002/2016JD025224 |
Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility | |
LePage, Daniel P.1; Metcalf, Jason A.1; Bordenstein, Sarah R.1; On, Jungmin1; Perlmutter, Jessamyn I.1; Shropshire, J. Dylan1; Layton, Emily M.1; Funkhouser-Jones, Lisa J.1; Beckmann, John F.2; Bordenstein, Seth R.1,3 | |
2017-03-09 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 543期号:7644页码:243-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The genus Wolbachia is an archetype of maternally inherited intracellular bacteria that infect the germline of numerous invertebrate species worldwide. They can selfishly alter arthropod sex ratios and reproductive strategies to increase the proportion of the infected matriline in the population. The most common reproductive manipulation is cytoplasmic incompatibility, which results in embryonic lethality in crosses between infected males and uninfected females. Females infected with the same Wolbachia strain rescue this lethality. Despite more than 40 years of research(1) and relevance to symbiont-induced speciation(2,3), as well as control of arbovirus vectors(4-6) and agricultural pests(7), the bacterial genes underlying cytoplasmic incompatibility remain unknown. Here we use comparative and transgenic approaches to demonstrate that two differentially transcribed, co-diverging genes in the eukaryotic association module of prophage WO8 from Wolbachia strain wMel recapitulate and enhance cytoplasmic incompatibility. Dual expression in transgenic, uninfected males of Drosophila melanogaster crossed to uninfected females causes embryonic lethality. Each gene additively augments embryonic lethality in crosses between infected males and uninfected females. Lethality associates with embryonic defects that parallel those of wild-type cytoplasmic incompatibility and is notably rescued by wMel-infected embryos in all cases. The discovery of cytoplasmic incompatibility factor genes cifA and cifB pioneers genetic studies of prophage WO-induced reproductive manipulations and informs the continuing use of Wolbachia to control dengue and Zika virus transmission to humans. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000395688700039 |
WOS关键词 | DROSOPHILA-SIMULANS ; PHAGE WO ; PROTEIN ; STRAIN ; MELANOGASTER ; EXPRESSION ; EVOLUTION ; MOSQUITOS ; DATABASE ; GENOMES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/32197 |
专题 | 气候变化 |
作者单位 | 1.Vanderbilt Univ, Dept Biol Sci, 221 Kirkland Hall, Nashville, TN 37235 USA; 2.Yale Univ, Dept Mol Biophys & Biochem, POB 6666, New Haven, CT 06520 USA; 3.Vanderbilt Univ, Dept Pathol Microbiol & Immunol, 221 Kirkland Hall, Nashville, TN 37235 USA |
推荐引用方式 GB/T 7714 | LePage, Daniel P.,Metcalf, Jason A.,Bordenstein, Sarah R.,et al. Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility[J]. NATURE,2017,543(7644):243-+. |
APA | LePage, Daniel P..,Metcalf, Jason A..,Bordenstein, Sarah R..,On, Jungmin.,Perlmutter, Jessamyn I..,...&Bordenstein, Seth R..(2017).Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility.NATURE,543(7644),243-+. |
MLA | LePage, Daniel P.,et al."Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility".NATURE 543.7644(2017):243-+. |
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