Landmarks of human embryonic development inscribed in somatic mutations

doi:10.1126/science.abe1544

GSTDTAP > 气候变化

DOI	10.1126/science.abe1544
	Landmarks of human embryonic development inscribed in somatic mutations
	Sara Bizzotto; Yanmei Dou; Javier Ganz; Ryan N. Doan; Minseok Kwon; Craig L. Bohrson; Sonia N. Kim; Taejeong Bae; Alexej Abyzov; NIMH Brain Somatic Mosaicism Network†; Peter J. Park; Christopher A. Walsh
	2021-03-19
发表期刊	Science
出版年	2021
英文摘要	Somatic mutations pepper our cells with change, but because they are not in the germline, they do not propagate to the next generation. Bizzotto et al. leveraged data on the distribution of somatic mutations in adults to take a backward look at the earliest moments of human development. Calculation of cellular lineages on the basis of shared somatic mutations shows the number of cells from which the body will develop when the human embryo gastrulates. The lineage for forebrain cells is identifiable, as are the asymmetrical fates spun out of many of the gastrula cells. Science , this issue p. [1249][1] Although cell lineage information is fundamental to understanding organismal development, very little direct information is available for humans. We performed high-depth (250×) whole-genome sequencing of multiple tissues from three individuals to identify hundreds of somatic single-nucleotide variants (sSNVs). Using these variants as “endogenous barcodes” in single cells, we reconstructed early embryonic cell divisions. Targeted sequencing of clonal sSNVs in different organs (about 25,000×) and in more than 1000 cortical single cells, as well as single-nucleus RNA sequencing and single-nucleus assay for transposase-accessible chromatin sequencing of ~100,000 cortical single cells, demonstrated asymmetric contributions of early progenitors to extraembryonic tissues, distinct germ layers, and organs. Our data suggest onset of gastrulation at an effective progenitor pool of about 170 cells and about 50 to 100 founders for the forebrain. Thus, mosaic mutations provide a permanent record of human embryonic development at very high resolution. [1]: /lookup/doi/10.1126/science.abe1544
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/319937
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Sara Bizzotto,Yanmei Dou,Javier Ganz,et al. Landmarks of human embryonic development inscribed in somatic mutations[J]. Science,2021.
APA	Sara Bizzotto.,Yanmei Dou.,Javier Ganz.,Ryan N. Doan.,Minseok Kwon.,...&Christopher A. Walsh.(2021).Landmarks of human embryonic development inscribed in somatic mutations.Science.
MLA	Sara Bizzotto,et al."Landmarks of human embryonic development inscribed in somatic mutations".Science (2021).