Processive RNA polymerization and promoter recognition in an RNA World

doi:10.1126/science.abd9191

GSTDTAP > 气候变化

DOI	10.1126/science.abd9191
	Processive RNA polymerization and promoter recognition in an RNA World
	Razvan Cojocaru; Peter J. Unrau
	2021-03-19
发表期刊	Science
出版年	2021
英文摘要	The RNA World Hypothesis suggests that, before modern life, there were RNA molecules that were capable of carrying genetic information and driving chemical reactions, a task gradually replaced by DNA and enzymes in modern biology. Central to this theory is an RNA replicase capable of mediating general replication of RNA. Using laboratory evolution, Cojocaru et al. isolated a promoter-based RNA polymerase ribozyme that, analogous to modern-day protein polymerases, clamps onto templates to increase its processivity, making it a potential model for replication in early biology. Science , this issue p. [1225][1] Early life is thought to have required the self-replication of RNA by RNA replicases. However, how such replicases evolved and subsequently enabled gene expression remains largely unexplored. We engineered and selected a holopolymerase ribozyme that uses a sigma factor–like specificity primer to first recognize an RNA promoter sequence and then, in a second step, rearrange to a processive elongation form. Using its own sequence, the polymerase can also program itself to polymerize from certain RNA promoters and not others. This selective promoter–based polymerization could allow an RNA replicase ribozyme to define “self” from “nonself,” an important development for the avoidance of replicative parasites. Moreover, the clamp-like mechanism of this polymerase could eventually enable strand invasion, a critical requirement for replication in the early evolution of life. [1]: /lookup/doi/10.1126/science.abd9191
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/319932
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Razvan Cojocaru,Peter J. Unrau. Processive RNA polymerization and promoter recognition in an RNA World[J]. Science,2021.
APA	Razvan Cojocaru,&Peter J. Unrau.(2021).Processive RNA polymerization and promoter recognition in an RNA World.Science.
MLA	Razvan Cojocaru,et al."Processive RNA polymerization and promoter recognition in an RNA World".Science (2021).