Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice

doi:10.1126/science.abf6840

GSTDTAP > 气候变化

DOI	10.1126/science.abf6840
	Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
	Alexander A. Cohen; Priyanthi N. P. Gnanapragasam; Yu E. Lee; Pauline R. Hoffman; Susan Ou; Leesa M. Kakutani; Jennifer R. Keeffe; Hung-Jen Wu; Mark Howarth; Anthony P. West; Christopher O. Barnes; Michel C. Nussenzweig; Pamela J. Bjorkman
	2021-02-12
发表期刊	Science
出版年	2021
英文摘要	In the past 20 years, three betacoronaviruses thought to have originated in bats have caused devastating disease in humans. The global pandemic caused by the latest such virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the need to protect against other strains that could present a threat to humans. Cohen et al. constructed nanoparticles displaying the protein domain that binds the host cell receptor (receptor-binding domain or RBD), either a homotypic SARS-CoV-2 particle or mosaic particles displaying RBDs from four or eight different betacoronaviruses. In mice, antibodies to the SARS-CoV-2 RBD were elicited just as well by mosaic particles as by homotypic nanoparticles. The mosaic nanoparticles elicited antibodies that, beyond recognizing the strains displayed, also recognized mismatched strains. Science , this issue p. [735][1] Protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and SARS-related emergent zoonotic coronaviruses is urgently needed. We made homotypic nanoparticles displaying the receptor binding domain (RBD) of SARS-CoV-2 or co-displaying SARS-CoV-2 RBD along with RBDs from animal betacoronaviruses that represent threats to humans (mosaic nanoparticles with four to eight distinct RBDs). Mice immunized with RBD nanoparticles, but not soluble antigen, elicited cross-reactive binding and neutralization responses. Mosaic RBD nanoparticles elicited antibodies with superior cross-reactive recognition of heterologous RBDs relative to sera from immunizations with homotypic SARS-CoV-2–RBD nanoparticles or COVID-19 convalescent human plasmas. Moreover, after priming, sera from mosaic RBD–immunized mice neutralized heterologous pseudotyped coronaviruses as well as or better than sera from homotypic SARS-CoV-2–RBD nanoparticle immunizations, demonstrating no loss of immunogenicity against particular RBDs resulting from co-display. A single immunization with mosaic RBD nanoparticles provides a potential strategy to simultaneously protect against SARS-CoV-2 and emerging zoonotic coronaviruses. [1]: /lookup/doi/10.1126/science.abf6840
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/314093
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Alexander A. Cohen,Priyanthi N. P. Gnanapragasam,Yu E. Lee,et al. Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice[J]. Science,2021.
APA	Alexander A. Cohen.,Priyanthi N. P. Gnanapragasam.,Yu E. Lee.,Pauline R. Hoffman.,Susan Ou.,...&Pamela J. Bjorkman.(2021).Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice.Science.
MLA	Alexander A. Cohen,et al."Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice".Science (2021).