Structure of a transcribing RNA polymerase II–U1 snRNP complex

doi:10.1126/science.abf1870

GSTDTAP > 气候变化

DOI	10.1126/science.abf1870
	Structure of a transcribing RNA polymerase II–U1 snRNP complex
	Suyang Zhang; Shintaro Aibara; Seychelle M. Vos; Dmitry E. Agafonov; Reinhard Lührmann; Patrick Cramer
	2021-01-15
发表期刊	Science
出版年	2021
英文摘要	Gene expression in eukaryotes first requires transcription of DNA to an RNA copy and then splicing to form the final, processed messenger RNA (mRNA). Zhang et al. investigated how gene transcription and RNA splicing are physically coupled. Using cryo–electron microscopy, they resolved the molecular structure of a complex of the transcription enzyme RNA polymerase II with part of the splicing machinery, the U1 small nuclear ribonucleoprotein particle. The results provide important details for our understanding of coupled mRNA production. Science , this issue p. [305][1] To initiate cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA). Here, we report the cryo–electron microscopy structure of a mammalian transcribing Pol II–U1 snRNP complex. The structure reveals that Pol II and U1 snRNP interact directly. This interaction positions the pre-mRNA 5′ splice site near the RNA exit site of Pol II. Extension of pre-mRNA retains the 5′ splice site, leading to the formation of a “growing intron loop.” Loop formation may facilitate scanning of nascent pre-mRNA for the 3′ splice site, functional pairing of distant intron ends, and prespliceosome assembly. Our results provide a starting point for a mechanistic analysis of cotranscriptional spliceosome assembly and the biogenesis of mRNA isoforms by alternative splicing. [1]: /lookup/doi/10.1126/science.abf1870
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/311511
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Suyang Zhang,Shintaro Aibara,Seychelle M. Vos,等. Structure of a transcribing RNA polymerase II–U1 snRNP complex[J]. Science,2021.
APA	Suyang Zhang,Shintaro Aibara,Seychelle M. Vos,Dmitry E. Agafonov,Reinhard Lührmann,&Patrick Cramer.(2021).Structure of a transcribing RNA polymerase II–U1 snRNP complex.Science.
MLA	Suyang Zhang,et al."Structure of a transcribing RNA polymerase II–U1 snRNP complex".Science (2021).