Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.2016380117 |
Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soil Pseudomonas species | |
Caroll M. Mendonca; Sho Yoshitake; Hua Wei; Anne Werner; Samantha S. Sasnow; Theodore W. Thannhauser; Ludmilla Aristilde | |
2020-12-03 | |
发表期刊 | Proceedings of the National Academy of Sciences
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出版年 | 2020 |
英文摘要 | High-affinity iron (Fe) scavenging compounds, or siderophores, are widely employed by soil bacteria to survive scarcity in bioavailable Fe. Siderophore biosynthesis relies on cellular carbon metabolism, despite reported decrease in both carbon uptake and Fe-containing metabolic proteins in Fe-deficient cells. Given this paradox, the metabolic network required to sustain the Fe-scavenging strategy is poorly understood. Here, through multiple 13C-metabolomics experiments with Fe-replete and Fe-limited cells, we uncover how soil Pseudomonas species reprogram their metabolic pathways to prioritize siderophore biosynthesis. Across the three species investigated (Pseudomonas putida KT2440, Pseudomonas protegens Pf-5, and Pseudomonas putida S12), siderophore secretion is higher during growth on gluconeogenic substrates than during growth on glycolytic substrates. In response to Fe limitation, we capture decreased flux toward the tricarboxylic acid (TCA) cycle during the metabolism of glycolytic substrates but, due to carbon recycling to the TCA cycle via enhanced anaplerosis, the metabolism of gluconeogenic substrates results in an increase in both siderophore secretion (up to threefold) and Fe extraction (up to sixfold) from soil minerals. During simultaneous feeding on the different substrate types, Fe deficiency triggers a hierarchy in substrate utilization, which is facilitated by changes in protein abundances for substrate uptake and initial catabolism. Rerouted metabolism further promotes favorable fluxes in the TCA cycle and the gluconeogenesis–anaplerosis nodes, despite decrease in several proteins in these pathways, to meet carbon and energy demands for siderophore precursors in accordance with increased proteins for siderophore biosynthesis. Hierarchical carbon metabolism thus serves as a critical survival strategy during the metal nutrient deficiency. |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/305796 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Caroll M. Mendonca,Sho Yoshitake,Hua Wei,et al. Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soil Pseudomonas species[J]. Proceedings of the National Academy of Sciences,2020. |
APA | Caroll M. Mendonca.,Sho Yoshitake.,Hua Wei.,Anne Werner.,Samantha S. Sasnow.,...&Ludmilla Aristilde.(2020).Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soil Pseudomonas species.Proceedings of the National Academy of Sciences. |
MLA | Caroll M. Mendonca,et al."Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soil Pseudomonas species".Proceedings of the National Academy of Sciences (2020). |
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