Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms

doi:10.1126/science.abe3354

GSTDTAP > 气候变化

DOI	10.1126/science.abe3354
	Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms
	M. Alejandra Tortorici; Martina Beltramello; Florian A. Lempp; Dora Pinto; Ha V. Dang; Laura E. Rosen; Matthew McCallum; John Bowen; Andrea Minola; Stefano Jaconi; Fabrizia Zatta; Anna De Marco; Barbara Guarino; Siro Bianchi; Elvin J. Lauron; Heather Tucker; Jiayi Zhou; Alessia Peter; Colin Havenar-Daughton; Jason A. Wojcechowskyj; James Brett Case; Rita E. Chen; Hannah Kaiser; Martin Montiel-Ruiz; Marcel Meury; Nadine Czudnochowski; Roberto Spreafico; Josh Dillen; Cindy Ng; Nicole Sprugasci; Katja Culap; Fabio Benigni; Rana Abdelnabi; Shi-Yan Caroline Foo; Michael A. Schmid; Elisabetta Cameroni; Agostino Riva; Arianna Gabrieli; Massimo Galli; Matteo S. Pizzuto; Johan Neyts; Michael S. Diamond; Herbert W. Virgin; Gyorgy Snell; Davide Corti; Katja Fink; David Veesler
	2020-11-20
发表期刊	Science
出版年	2020
英文摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is initiated by the trimeric spike protein that decorates the virus and binds the ACE2 receptor. Antibodies against the spike that neutralize viral infection have potential as therapeutics. Tortorici et al. describe two very potent antibodies, S2E12 and S2M11. Electron microscopy structures characterized the binding and showed that S2E12 traps the spike in a conformation that cannot bind ACE2. Both antibodies protected hamsters against SARS-CoV-2 challenge and may be useful in antibody cocktails to combat the virus and prevent the development of resistance. Science , this issue p. [950][1] Efficient therapeutic options are needed to control the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that has caused more than 922,000 fatalities as of 13 September 2020. We report the isolation and characterization of two ultrapotent SARS-CoV-2 human neutralizing antibodies (S2E12 and S2M11) that protect hamsters against SARS-CoV-2 challenge. Cryo–electron microscopy structures show that S2E12 and S2M11 competitively block angiotensin-converting enzyme 2 (ACE2) attachment and that S2M11 also locks the spike in a closed conformation by recognition of a quaternary epitope spanning two adjacent receptor-binding domains. Antibody cocktails that include S2M11, S2E12, or the previously identified S309 antibody broadly neutralize a panel of circulating SARS-CoV-2 isolates and activate effector functions. Our results pave the way to implement antibody cocktails for prophylaxis or therapy, circumventing or limiting the emergence of viral escape mutants. [1]: /lookup/doi/10.1126/science.abe3354
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/304401
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	M. Alejandra Tortorici,Martina Beltramello,Florian A. Lempp,et al. Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms[J]. Science,2020.
APA	M. Alejandra Tortorici.,Martina Beltramello.,Florian A. Lempp.,Dora Pinto.,Ha V. Dang.,...&David Veesler.(2020).Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms.Science.
MLA	M. Alejandra Tortorici,et al."Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms".Science (2020).