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DOI | 10.1289/EHP7058 |
Effects of PCB126 on Adipose-to-Muscle Communication in an in Vitro Model | |
Audrey Caron; Fozia Ahmed; Vian Peshdary; Léa Garneau; Ella Atlas; Céline Aguer | |
2020-10-07 | |
发表期刊 | Environmental Health Perspectives |
出版年 | 2020 |
英文摘要 | AbstractBackground:Exposure to coplanar polychlorinated biphenyls (PCBs) is linked to the development of insulin resistance. Previous studies suggested PCB126 alters muscle mitochondrial function through an indirect mechanism. Given that PCBs are stored in fat, we hypothesized that PCB126 alters adipokine secretion, which in turn affects muscle metabolism. Objectives:We determined a) the impacts of PCB126 exposure on adipocyte cytokine/adipokine secretion in vitro; b) whether adipocyte-derived factors alter glucose metabolism and mitochondrial function in myotubes when exposed to PCB126; and c) whether preestablished insulin resistance alters the metabolic responses of adipocytes exposed to PCB126 and the communication between adipocytes and myotubes. Methods:3T3-L1 adipocytes were exposed to PCB126 () in two insulin sensitivity conditions [insulin sensitive (IS) and insulin resistant (IR) adipocytes], followed by the measurement of secreted adipokines, mitochondrial function, and insulin-stimulated glucose uptake. Communication between adipocytes and myotubes was reproduced by exposing C2C12 myotubes or mouse primary myotubes to conditioned medium (CM) derived from IS or IR 3T3-L1 adipocytes exposed to PCB126. Mitochondrial function and insulin-stimulated glucose uptake were then determined in myotubes. Results:IR 3T3-L1 adipocytes treated with PCB126 had significantly higher adipokine (adiponectin, IL-6, MCP-1, ) secretion and lower mitochondrial function, glucose uptake, and glycolysis. However, PCB126 did not significantly alter these parameters in IS adipocytes. Altered energy metabolism in IR 3T3-L1 adipocytes was linked to lower phosphorylation of AMP-activated protein kinase (p-AMPK) and higher superoxide dismutase 2 levels, an enzyme involved in reactive oxygen species detoxification. Myotubes exposed to the CM from PCB126-treated IR adipocytes had lower glucose uptake, with no alteration in glycolysis or mitochondrial function. Interestingly, p-AMPK levels were higher in myotubes exposed to the CM of PCB126-treated IR adipocytes. Discussion:Taken together, these data suggest that increased adipokine secretion from IR adipocytes exposed to PCB126 might explain impaired glucose uptake in myotubes. https://doi.org/10.1289/EHP7058 |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/297774 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Audrey Caron,Fozia Ahmed,Vian Peshdary,et al. Effects of PCB126 on Adipose-to-Muscle Communication in an in Vitro Model[J]. Environmental Health Perspectives,2020. |
APA | Audrey Caron,Fozia Ahmed,Vian Peshdary,Léa Garneau,Ella Atlas,&Céline Aguer.(2020).Effects of PCB126 on Adipose-to-Muscle Communication in an in Vitro Model.Environmental Health Perspectives. |
MLA | Audrey Caron,et al."Effects of PCB126 on Adipose-to-Muscle Communication in an in Vitro Model".Environmental Health Perspectives (2020). |
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