Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy

doi:10.1126/science.abc4730

GSTDTAP > 气候变化

DOI	10.1126/science.abc4730
	Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
	Hongjing Gu; Qi Chen; Guan Yang; Lei He; Hang Fan; Yong-Qiang Deng; Yanxiao Wang; Yue Teng; Zhongpeng Zhao; Yujun Cui; Yuchang Li; Xiao-Feng Li; Jiangfan Li; Na-Na Zhang; Xiaolan Yang; Shaolong Chen; Yan Guo; Guangyu Zhao; Xiliang Wang; De-Yan Luo; Hui Wang; Xiao Yang; Yan Li; Gencheng Han; Yuxian He; Xiaojun Zhou; Shusheng Geng; Xiaoli Sheng; Shibo Jiang; Shihui Sun; Cheng-Feng Qin; Yusen Zhou
	2020-09-25
发表期刊	Science
出版年	2020
英文摘要	Among the research tools necessary to develop medical interventions to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, high on the list are informative animal models with which to study viral pathogenesis. Gu et al. developed a mouse model in which a SARS-CoV-2 strain was infectious and could cause an inflammatory response and moderate pneumonia. Adaptation of this viral strain in the mouse appeared to be dependent on a critical amino acid change, Asn501 to Tyr (N501Y), within the receptor-binding domain of the viral spike protein. The new mouse model was used to study neutralizing antibodies and a vaccine candidate against the virus. Science , this issue p. [1603][1] The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the respiratory tract of aged BALB/c mice. The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. The protective efficacy of a recombinant RBD vaccine candidate was validated by using this model. Thus, this mouse-adapted strain and associated challenge model should be of value in evaluating vaccines and antivirals against SARS-CoV-2. [1]: /lookup/doi/10.1126/science.abc4730
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/296506
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Hongjing Gu,Qi Chen,Guan Yang,et al. Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy[J]. Science,2020.
APA	Hongjing Gu.,Qi Chen.,Guan Yang.,Lei He.,Hang Fan.,...&Yusen Zhou.(2020).Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy.Science.
MLA	Hongjing Gu,et al."Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy".Science (2020).