Cryo-EM structure of 90<em>S</em> small ribosomal subunit precursors in transition states

doi:10.1126/science.aba9690

GSTDTAP > 气候变化

DOI	10.1126/science.aba9690
	Cryo-EM structure of 90S small ribosomal subunit precursors in transition states
	Yifei Du; Weidong An; Xing Zhu; Qi Sun; Jia Qi; Keqiong Ye
	2020-09-18
发表期刊	Science
出版年	2020
英文摘要	The formation of eukaryotic ribosomes is a complex process that starts with transcription of a large precursor RNA that assembles into a large 90S preribosome, which matures to finally give the 40S small subunit of the ribosome. Cheng et al. and Du et al. give insight into this process, using cryo–electron microscopy to look at intermediates along the pathway. Together, these studies reveal how a cast of molecular players act to coordinate the compositional and structural changes that transform the 90S preribosome into a pre-40S subunit. Science , this issue p. [1470][1], p. [1477][2] The 90 S preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 S ribosome. Here, we gained insight into this transition by determining cryo–electron microscopy structures of Saccharomyces cerevisiae intermediates in the path from the 90 S to the pre-40 S . The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5′ external transcribed spacer (5′ ETS) is degraded within 90 S , driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 S through helicase Mtr4 and is primed to digest the 3′ end of the 5′ ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5′ ETS degradation in 90 S progression. [1]: /lookup/doi/10.1126/science.abb4119 [2]: /lookup/doi/10.1126/science.aba9690
领域	气候变化 ; 资源环境
URL	查看原文
引用统计	被引频次：51[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/295426
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Yifei Du,Weidong An,Xing Zhu,et al. Cryo-EM structure of 90S small ribosomal subunit precursors in transition states[J]. Science,2020.
APA	Yifei Du,Weidong An,Xing Zhu,Qi Sun,Jia Qi,&Keqiong Ye.(2020).Cryo-EM structure of 90S small ribosomal subunit precursors in transition states.Science.
MLA	Yifei Du,et al."Cryo-EM structure of 90S small ribosomal subunit precursors in transition states".Science (2020).