90<em>S</em> pre-ribosome transformation into the primordial 40<em>S</em> subunit

doi:10.1126/science.abb4119

GSTDTAP > 气候变化

DOI	10.1126/science.abb4119
	90S pre-ribosome transformation into the primordial 40S subunit
	Jingdong Cheng; Benjamin Lau; Giuseppe La Venuta; Michael Ameismeier; Otto Berninghausen; Ed Hurt; Roland Beckmann
	2020-09-18
发表期刊	Science
出版年	2020
英文摘要	The formation of eukaryotic ribosomes is a complex process that starts with transcription of a large precursor RNA that assembles into a large 90S preribosome, which matures to finally give the 40S small subunit of the ribosome. Cheng et al. and Du et al. give insight into this process, using cryo–electron microscopy to look at intermediates along the pathway. Together, these studies reveal how a cast of molecular players act to coordinate the compositional and structural changes that transform the 90S preribosome into a pre-40S subunit. Science , this issue p. [1470][1], p. [1477][2] Production of small ribosomal subunits initially requires the formation of a 90 S precursor followed by an enigmatic process of restructuring into the primordial pre-40 S subunit. We elucidate this process by biochemical and cryo–electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 S pre-ribosome, followed by Utp24 endonuclease–driven RNA cleavage at site A1, thereby separating the 5′-external transcribed spacer (ETS) from 18 S ribosomal RNA. Next, the 5′-ETS and 90 S assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 S emerges, still retaining some 90 S factors including Dhr1, now ready to unwind the final small nucleolar U3–18 S RNA hybrid. Our data shed light on the elusive 90 S to pre-40 S transition and clarify the principles of assembly and remodeling of large ribonucleoproteins. [1]: /lookup/doi/10.1126/science.abb4119 [2]: /lookup/doi/10.1126/science.aba9690
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/295425
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jingdong Cheng,Benjamin Lau,Giuseppe La Venuta,et al. 90S pre-ribosome transformation into the primordial 40S subunit[J]. Science,2020.
APA	Jingdong Cheng.,Benjamin Lau.,Giuseppe La Venuta.,Michael Ameismeier.,Otto Berninghausen.,...&Roland Beckmann.(2020).90S pre-ribosome transformation into the primordial 40S subunit.Science.
MLA	Jingdong Cheng,et al."90S pre-ribosome transformation into the primordial 40S subunit".Science (2020).