Structure of a human 48<em>S</em> translational initiation complex

doi:10.1126/science.aba4904

GSTDTAP > 气候变化

DOI	10.1126/science.aba4904
	Structure of a human 48S translational initiation complex
	Jailson Brito Querido; Masaaki Sokabe; Sebastian Kraatz; Yuliya Gordiyenko; J. Mark Skehel; Christopher S. Fraser; V. Ramakrishnan
	2020-09-04
发表期刊	Science
出版年	2020
英文摘要	Eukaryotic translation involves many players in a dynamic and well-orchestrated process. A 43 S preinitiation complex (PIC) comprises the 40 S ribosomal subunit; initiation factors, including the eIF3 complex, which is known to play a key role; and the transfer RNA used for translation initiation. The PIC is recruited to the cap-binding complex eIF4F at the 5′ end of messenger RNA (mRNA) to form a 48 S complex that scans along the mRNA for a start codon. Brito Querido et al. determined the structure of a reconstituted human 48 S complex using cryo–electron microscopy. They found that eIF4F binds to eIF3 near the exit site of the ribosome. This positioning suggests that downstream mRNA is likely pulled through the 40 S subunit to find the start codon. Science , this issue p. [1220][1] A key step in translational initiation is the recruitment of the 43 S preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5′ end of messenger RNA (mRNA) to form the 48 S initiation complex (i.e., the 48 S ). The 48 S then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo–electron microscopy to determine the structure of a reconstituted human 48 S . The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 S . The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being “pulled” through the 40 S subunit during scanning. [1]: /lookup/doi/10.1126/science.aba4904
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/293269
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jailson Brito Querido,Masaaki Sokabe,Sebastian Kraatz,et al. Structure of a human 48S translational initiation complex[J]. Science,2020.
APA	Jailson Brito Querido.,Masaaki Sokabe.,Sebastian Kraatz.,Yuliya Gordiyenko.,J. Mark Skehel.,...&V. Ramakrishnan.(2020).Structure of a human 48S translational initiation complex.Science.
MLA	Jailson Brito Querido,et al."Structure of a human 48S translational initiation complex".Science (2020).