Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans

doi:10.1126/science.abc6261

GSTDTAP > 气候变化

DOI	10.1126/science.abc6261
	Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans
	Prabhu S. Arunachalam; Florian Wimmers; Chris Ka Pun Mok; Ranawaka A. P. M. Perera; Madeleine Scott; Thomas Hagan; Natalia Sigal; Yupeng Feng; Laurel Bristow; Owen Tak-Yin Tsang; Dhananjay Wagh; John Coller; Kathryn L. Pellegrini; Dmitri Kazmin; Ghina Alaaeddine; Wai Shing Leung; Jacky Man Chun Chan; Thomas Shiu Hong Chik; Chris Yau Chung Choi; Christopher Huerta; Michele Paine McCullough; Huibin Lv; Evan Anderson; Srilatha Edupuganti; Amit A. Upadhyay; Steve E. Bosinger; Holden Terry Maecker; Purvesh Khatri; Nadine Rouphael; Malik Peiris; Bali Pulendran
	2020-09-04
发表期刊	Science
出版年	2020
英文摘要	Coronavirus disease 2019 (COVID-19) has affected millions of people globally, yet how the human immune system responds to and influences COVID-19 severity remains unclear. Mathew et al. present a comprehensive atlas of immune modulation associated with COVID-19. They performed high-dimensional flow cytometry of hospitalized COVID-19 patients and found three prominent and distinct immunotypes that are related to disease severity and clinical parameters. Arunachalam et al. report a systems biology approach to assess the immune system of COVID-19 patients with mild-to-severe disease. These studies provide a compendium of immune cell information and roadmaps for potential therapeutic interventions. Science , this issue p. [eabc8511][1], p. [1210][2] Coronavirus disease 2019 (COVID-19) represents a global crisis, yet major knowledge gaps remain about human immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We analyzed immune responses in 76 COVID-19 patients and 69 healthy individuals from Hong Kong and Atlanta, Georgia, United States. In the peripheral blood mononuclear cells (PBMCs) of COVID-19 patients, we observed reduced expression of human leukocyte antigen class DR (HLA-DR) and proinflammatory cytokines by myeloid cells as well as impaired mammalian target of rapamycin (mTOR) signaling and interferon-α (IFN-α) production by plasmacytoid dendritic cells. By contrast, we detected enhanced plasma levels of inflammatory mediators—including EN-RAGE, TNFSF14, and oncostatin M—which correlated with disease severity and increased bacterial products in plasma. Single-cell transcriptomics revealed a lack of type I IFNs, reduced HLA-DR in the myeloid cells of patients with severe COVID-19, and transient expression of IFN-stimulated genes. This was consistent with bulk PBMC transcriptomics and transient, low IFN-α levels in plasma during infection. These results reveal mechanisms and potential therapeutic targets for COVID-19. [1]: /lookup/doi/10.1126/science.abc8511 [2]: /lookup/doi/10.1126/science.abc6261
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/293268
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Prabhu S. Arunachalam,Florian Wimmers,Chris Ka Pun Mok,et al. Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans[J]. Science,2020.
APA	Prabhu S. Arunachalam.,Florian Wimmers.,Chris Ka Pun Mok.,Ranawaka A. P. M. Perera.,Madeleine Scott.,...&Bali Pulendran.(2020).Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans.Science.
MLA	Prabhu S. Arunachalam,et al."Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans".Science (2020).