Mosquito cellular immunity at single-cell resolution

doi:10.1126/science.abc0322

GSTDTAP > 气候变化

DOI	10.1126/science.abc0322
	Mosquito cellular immunity at single-cell resolution
	Gianmarco Raddi; Ana Beatriz F. Barletta; Mirjana Efremova; Jose Luis Ramirez; Rafael Cantera; Sarah A. Teichmann; Carolina Barillas-Mury; Oliver Billker
	2020-08-28
发表期刊	Science
出版年	2020
英文摘要	Hemocytes are key immune cells of insects, playing a major role in how vector species transmit disease. Raddi et al. collected and sequenced the RNA of more than 8000 individual hemocytes from the disease-carrying mosquitoes Anopheles gambiae and Aedes aegypti . These data were then analyzed to determine hemocyte differentiation lineages and population changes in response to noninfected and Plasmodium -infected blood meals. From these data, the researchers identified a new hemocyte type, the megacyte, defined by the transcription of specific marker genes. Gene-silencing experiments implied that the megacyte functions in hemocyte differentiation during immune priming and thus may be involved in the immune response in mosquitoes. Science , this issue p. [1128][1] Hemocytes limit the capacity of mosquitoes to transmit human pathogens. Here we profile the transcriptomes of 8506 hemocytes of Anopheles gambiae and Aedes aegypti mosquito vectors. Our data reveal the functional diversity of hemocytes, with different subtypes of granulocytes expressing distinct and evolutionarily conserved subsets of effector genes. A previously unidentified cell type in An. gambiae , which we term “megacyte,” is defined by a specific transmembrane protein marker (TM7318) and high expression of lipopolysaccharide-induced tumor necrosis factor–α transcription factor 3 (LL3). Knockdown experiments indicate that LL3 mediates hemocyte differentiation during immune priming. We identify and validate two main hemocyte lineages and find evidence of proliferating granulocyte populations. This atlas of medically relevant invertebrate immune cells at single-cell resolution identifies cellular events that underpin mosquito immunity to malaria infection. [1]: /lookup/doi/10.1126/science.abc0322
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/293234
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Gianmarco Raddi,Ana Beatriz F. Barletta,Mirjana Efremova,et al. Mosquito cellular immunity at single-cell resolution[J]. Science,2020.
APA	Gianmarco Raddi.,Ana Beatriz F. Barletta.,Mirjana Efremova.,Jose Luis Ramirez.,Rafael Cantera.,...&Oliver Billker.(2020).Mosquito cellular immunity at single-cell resolution.Science.
MLA	Gianmarco Raddi,et al."Mosquito cellular immunity at single-cell resolution".Science (2020).