In-cell architecture of an actively transcribing-translating expressome

doi:10.1126/science.abb3758

GSTDTAP > 气候变化

DOI	10.1126/science.abb3758
	In-cell architecture of an actively transcribing-translating expressome
	Francis J. O’Reilly; Liang Xue; Andrea Graziadei; Ludwig Sinn; Swantje Lenz; Dimitry Tegunov; Cedric Blötz; Neil Singh; Wim J. H. Hagen; Patrick Cramer; Jörg Stülke; Julia Mahamid; Juri Rappsilber
	2020-07-31
发表期刊	Science
出版年	2020
英文摘要	In bacteria, RNA polymerases can associate with ribosomes to form transcription-translation units called expressomes. Multiple models based on structural data of in vitro reconstitution assays have been proposed for how the two machineries interface with one another. Understanding this bacteria-specific coupling mechanism offers insight regarding the central dogma of molecular biology and might be leveraged for antibiotic development. O'Reilly et al. found that the NusA protein interfaces between the two complexes. The authors combined cryo–electron tomography and cross-linking mass spectrometry to produce an integrative model of the transcribing, translating expressome of Mycoplasma pneumoniae obtained entirely from in-cell data. This approach contributes to the development of in-cell structural biology. Science , this issue p. [554][1] Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae . We combined whole-cell cross-linking mass spectrometry, cellular cryo–electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell. [1]: /lookup/doi/10.1126/science.abb3758
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/286869
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Francis J. O’Reilly,Liang Xue,Andrea Graziadei,et al. In-cell architecture of an actively transcribing-translating expressome[J]. Science,2020.
APA	Francis J. O’Reilly.,Liang Xue.,Andrea Graziadei.,Ludwig Sinn.,Swantje Lenz.,...&Juri Rappsilber.(2020).In-cell architecture of an actively transcribing-translating expressome.Science.
MLA	Francis J. O’Reilly,et al."In-cell architecture of an actively transcribing-translating expressome".Science (2020).