CRISPR-CasΦ from huge phages is a hypercompact genome editor

doi:10.1126/science.abb1400

GSTDTAP > 气候变化

DOI	10.1126/science.abb1400
	CRISPR-CasΦ from huge phages is a hypercompact genome editor
	Patrick Pausch; Basem Al-Shayeb; Ezra Bisom-Rapp; Connor A. Tsuchida; Zheng Li; Brady F. Cress; Gavin J. Knott; Steven E. Jacobsen; Jillian F. Banfield; Jennifer A. Doudna
	2020-07-17
发表期刊	Science
出版年	2020
英文摘要	The CRISPR-Cas system, naturally found in many prokaryotes, is widely used for genome editing. CRISPR arrays in the bacterial genome, derived from the genome of invading viruses, are used to generate a CRISPR RNA that guides the Cas enzyme to destroy repeat viral invaders. Recently, an unexpectedly compact CRISPR-Cas system was identified in huge bacteriophages. Pausch et al . show that even though this system lacks commonly found accessory proteins, it is functional. In addition to a CRISPR array, the only component of the system is an enzyme called CasF, which uses the same active site to process transcripts of the CRISPR arrays into CRISPR RNA and to destroy foreign nucleic acids. This system, which is active in human and plant cells, provides a hypercompact addition to the genome-editing toolbox. Science this issue p. [333][1] CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox. [1]: /lookup/doi/10.1126/science.abb1400
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/284357
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Patrick Pausch,Basem Al-Shayeb,Ezra Bisom-Rapp,et al. CRISPR-CasΦ from huge phages is a hypercompact genome editor[J]. Science,2020.
APA	Patrick Pausch.,Basem Al-Shayeb.,Ezra Bisom-Rapp.,Connor A. Tsuchida.,Zheng Li.,...&Jennifer A. Doudna.(2020).CRISPR-CasΦ from huge phages is a hypercompact genome editor.Science.
MLA	Patrick Pausch,et al."CRISPR-CasΦ from huge phages is a hypercompact genome editor".Science (2020).