Site-specific glycan analysis of the SARS-CoV-2 spike

doi:10.1126/science.abb9983

GSTDTAP > 气候变化

DOI	10.1126/science.abb9983
	Site-specific glycan analysis of the SARS-CoV-2 spike
	Yasunori Watanabe; Joel D. Allen; Daniel Wrapp; Jason S. McLellan; Max Crispin
	2020-07-17
发表期刊	Science
出版年	2020
英文摘要	Vaccine development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is focused on the trimeric spike protein that initiates infection. Each protomer in the trimeric spike has 22 glycosylation sites. How these sites are glycosylated may affect which cells the virus can infect and could shield some epitopes from antibody neutralization. Watanabe et al. expressed and purified recombinant glycosylated spike trimers, proteolysed them to yield glycopeptides containing a single glycan, and determined the composition of the glycan sites by mass spectrometry. The analysis provides a benchmark that can be used to measure antigen quality as vaccines and antibody tests are developed. Science this issue p. [330][1] The emergence of the betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), represents a considerable threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. The SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design. [1]: /lookup/doi/10.1126/science.abb9983
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/284356
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Yasunori Watanabe,Joel D. Allen,Daniel Wrapp,et al. Site-specific glycan analysis of the SARS-CoV-2 spike[J]. Science,2020.
APA	Yasunori Watanabe,Joel D. Allen,Daniel Wrapp,Jason S. McLellan,&Max Crispin.(2020).Site-specific glycan analysis of the SARS-CoV-2 spike.Science.
MLA	Yasunori Watanabe,et al."Site-specific glycan analysis of the SARS-CoV-2 spike".Science (2020).