Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy

doi:10.1126/science.abb8501

GSTDTAP > 气候变化

DOI	10.1126/science.abb8501
	Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy
	Boyang Qin; Chenyi Fei; Andrew A. Bridges; Ameya A. Mashruwala; Howard A. Stone; Ned S. Wingreen; Bonnie L. Bassler
	2020-07-03
发表期刊	Science
出版年	2020
英文摘要	Bacteria form three-dimensional communities called biofilms that are ubiquitous in nature and underlie human infections. Medically, biofilms are problematic because they protect resident cells from antibiotics. Although biofilms have been intensively studied, we do not understand how they develop cell by cell. Micron-sized bacteria are densely packed within biofilms, making it exceptionally challenging to track their movements. Qin et al. studied biofilm formation in the pathogen and model biofilm former Vibrio cholerae (see the Perspective by Dal Co and Brenner). The authors combined light-sheet microscopy with cell labeling to map the trajectories of a biofilm founder cell and its descendants in space and time as they built a biofilm. The findings revealed that as the bacteria reproduce, a bacterial “fountain” drives biofilm expansion and dictates the final positions of the offspring. Science , this issue p. [71][1]; see also p. [30][2] Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former Vibrio cholerae . It is unknown how spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion. We developed dual-view light-sheet microscopy to investigate the dynamics of biofilm development from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward, while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front, bypassing members trapped at the substrate and facilitating lateral biofilm expansion. This collective flow pattern was quantitatively captured by a continuum model of biofilm growth against substrate friction. Coordinated cell movement required the matrix protein RbmA, without which cells expanded erratically. Thus, tracking cell lineages and trajectories in space and time revealed how multicellular structures form from a single founder cell. [1]: /lookup/doi/10.1126/science.abb8501 [2]: /lookup/doi/10.1126/science.abd1225
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281883
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Boyang Qin,Chenyi Fei,Andrew A. Bridges,et al. Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy[J]. Science,2020.
APA	Boyang Qin.,Chenyi Fei.,Andrew A. Bridges.,Ameya A. Mashruwala.,Howard A. Stone.,...&Bonnie L. Bassler.(2020).Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy.Science.
MLA	Boyang Qin,et al."Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy".Science (2020).