A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity

doi:10.1126/science.abb6151

GSTDTAP > 气候变化

DOI	10.1126/science.abb6151
	A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity
	Alexander J. Meeske; Ning Jia; Alice K. Cassel; Albina Kozlova; Jingqiu Liao; Martin Wiedmann; Dinshaw J. Patel; Luciano A. Marraffini
	2020-07-03
发表期刊	Science
出版年	2020
英文摘要	CRISPR-Cas13 protects bacterial populations from viral infections by indiscriminately destroying the RNA of the cell and its invader, simultaneously arresting the growth of infected hosts and the spread of the virus. This response is mediated by the Cas13 nuclease, which unleashes massive RNA degradation after recognition of viral transcripts that are complementary to its guide RNA. Meeske et al. discovered AcrVIA1, a viral-encoded inhibitor that binds to Cas13 to occlude the RNA guide and prevent the activation of the nuclease (see the Perspective by Barrangou and Sontheimer). As opposed to inhibitors of DNA-cleaving CRISPR-Cas systems, which require multiple infections to neutralize all Cas nucleases of the host, production of AcrVIA1 by a single virus is sufficient to overcome the CRISPR-Cas13 response. Science , this issue p. [54][1]; see also p. [31][2] The CRISPR RNA (crRNA)–guided nuclease Cas13 recognizes complementary viral transcripts to trigger the degradation of both host and viral RNA during the type VI CRISPR-Cas antiviral response. However, how viruses can counteract this immunity is not known. We describe a listeriaphage (ϕLS46) encoding an anti-CRISPR protein (AcrVIA1) that inactivates the type VI-A CRISPR system of Listeria seeligeri . Using genetics, biochemistry, and structural biology, we found that AcrVIA1 interacts with the guide-exposed face of Cas13a, preventing access to the target RNA and the conformational changes required for nuclease activation. Unlike inhibitors of DNA-cleaving Cas nucleases, which cause limited immunosuppression and require multiple infections to bypass CRISPR defenses, a single dose of AcrVIA1 delivered by an individual virion completely dismantles type VI-A CRISPR-mediated immunity. [1]: /lookup/doi/10.1126/science.abb6151 [2]: /lookup/doi/10.1126/science.abc8243
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281875
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Alexander J. Meeske,Ning Jia,Alice K. Cassel,et al. A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity[J]. Science,2020.
APA	Alexander J. Meeske.,Ning Jia.,Alice K. Cassel.,Albina Kozlova.,Jingqiu Liao.,...&Luciano A. Marraffini.(2020).A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity.Science.
MLA	Alexander J. Meeske,et al."A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity".Science (2020).