SARS-CoV-2 productively infects human gut enterocytes

doi:10.1126/science.abc1669

GSTDTAP > 气候变化

DOI	10.1126/science.abc1669
	SARS-CoV-2 productively infects human gut enterocytes
	Mart M. Lamers; Joep Beumer; Jelte van der Vaart; Kèvin Knoops; Jens Puschhof; Tim I. Breugem; Raimond B. G. Ravelli; J. Paul van Schayck; Anna Z. Mykytyn; Hans Q. Duimel; Elly van Donselaar; Samra Riesebosch; Helma J. H. Kuijpers; Debby Schipper; Willine J. van de Wetering; Miranda de Graaf; Marion Koopmans; Edwin Cuppen; Peter J. Peters; Bart L. Haagmans; Hans Clevers
	2020-07-03
发表期刊	Science
出版年	2020
英文摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes an influenza-like disease with a respiratory transmission route; however, patients often present with gastrointestinal symptoms such as diarrhea, vomiting, and abdominal pain. Moreover, the virus has been detected in anal swabs, and cells in the inner-gut lining express the receptor that SARS-CoV-2 uses to gain entry to cells. Lamers et al. used human intestinal organoids, a “mini-gut” cultured in a dish, to demonstrate that SARS-CoV-2 readily replicates in an abundant cell type in the gut lining—the enterocyte—resulting in the production of large amounts of infective virus particles in the intestine. This work demonstrates that intestinal organoids can serve as a model to understand SARS-CoV-2 biology and infectivity in the gut. Science , this issue p. [50][1] Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology. [1]: /lookup/doi/10.1126/science.abc1669
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281873
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Mart M. Lamers,Joep Beumer,Jelte van der Vaart,et al. SARS-CoV-2 productively infects human gut enterocytes[J]. Science,2020.
APA	Mart M. Lamers.,Joep Beumer.,Jelte van der Vaart.,Kèvin Knoops.,Jens Puschhof.,...&Hans Clevers.(2020).SARS-CoV-2 productively infects human gut enterocytes.Science.
MLA	Mart M. Lamers,et al."SARS-CoV-2 productively infects human gut enterocytes".Science (2020).